NCALD (NM_001040630) Human Over-expression Lysate
Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 7
Product Datasheet for 'LY421789'
Specifications
Product Data | |
Description | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 7 |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC206321 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Accession Number | NM_001040630, NP_001035720 |
Other Names | MGC33870; MGC74858 |
Predicted MW | 22.3 kDa |
Components | 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT) |
Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -20°C. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20°C before shipping. |
Reference Data | |
RefSeq | NP_001035720 |
Locus ID | 83988 |
Cytogenetics | 8q22.3 |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
PH306321 | NCALD MS Standard C13 and N15-labeled recombinant protein (NP_001035720) |
USD 1,995.00 |
|
LC421785 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
LC421789 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
LC425719 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
TP306321 | Purified recombinant protein of Homo sapiens neurocalcin delta (NCALD), transcript variant 7 |
USD 748.00 |
|
LY425719 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 5 |
USD 315.00 |
|
TP314659 | Recombinant protein of human neurocalcin delta (NCALD), transcript variant 8 |
USD 748.00 |
|
PH314160 | NCALD MS Standard C13 and N15-labeled recombinant protein (NP_001035716) |
USD 1,995.00 |
|
LY421788 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 6 |
USD 315.00 |
|
LC421786 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
LY421783 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 1 |
USD 315.00 |
|
LY425717 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 3 |
USD 315.00 |
|
LY421785 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 3 |
USD 315.00 |
|
TP314160 | Purified recombinant protein of Homo sapiens neurocalcin delta (NCALD), transcript variant 3 |
USD 748.00 |
|
LC421788 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
LC410350 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
LC425716 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
LC421783 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
LY410350 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 8 |
USD 315.00 |
|
LC421787 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
PH312908 | NCALD MS Standard C13 and N15-labeled recombinant protein (NP_001035718) |
USD 1,995.00 |
|
AR09835PU-L | Recombinant human NCALD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
USD 820.00 |
|
PH314659 | NCALD MS Standard C13 and N15-labeled recombinant protein (NP_114430) |
USD 1,995.00 |
|
TP318434 | Purified recombinant protein of Homo sapiens neurocalcin delta (NCALD), transcript variant 6 |
USD 748.00 |
|
LY425718 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 4 |
USD 315.00 |
|
LY421787 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 5 |
USD 315.00 |
|
TP312908 | Purified recombinant protein of Homo sapiens neurocalcin delta (NCALD), transcript variant 5 |
USD 748.00 |
|
PH321428 | NCALD MS Standard C13 and N15-labeled recombinant protein (NP_001035714) |
USD 1,995.00 |
|
PH320693 | NCALD MS Standard C13 and N15-labeled recombinant protein (NP_001035717) |
USD 1,995.00 |
|
TP320693 | Purified recombinant protein of Homo sapiens neurocalcin delta (NCALD), transcript variant 4 |
USD 748.00 |
|
TP720212 | Recombinant protein of human neurocalcin delta (NCALD), transcript variant 1 |
USD 299.00 |
|
LC425720 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
LY425716 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 2 |
USD 315.00 |
|
LY421786 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 4 |
USD 315.00 |
|
LC425717 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
LY421784 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 2 |
USD 315.00 |
|
LC425715 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
TP313925 | Purified recombinant protein of Homo sapiens neurocalcin delta (NCALD), transcript variant 2 |
USD 748.00 |
|
LY425720 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 6 |
USD 315.00 |
|
PH318434 | NCALD MS Standard C13 and N15-labeled recombinant protein (NP_001035719) |
USD 1,995.00 |
|
AR09835PU-N | Recombinant human NCALD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
USD 320.00 |
|
LY425715 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 1 |
USD 315.00 |
|
LC425718 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
PH313925 | NCALD MS Standard C13 and N15-labeled recombinant protein (NP_001035715) |
USD 1,995.00 |
|
LY425721 | Transient overexpression lysate of neurocalcin delta (NCALD), transcript variant 7 |
USD 315.00 |
|
TP321428 | Purified recombinant protein of Homo sapiens neurocalcin delta (NCALD), transcript variant 1 |
USD 748.00 |
|
LC425721 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
LC421784 | NCALD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
USD 299.00
USD 360.00
USD 150.00