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NLRP3 (NM_001079821) Human Over-expression Lysate

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SKU
LY421550
Transient overexpression lysate of NLR family, pyrin domain containing 3 (NLRP3), transcript variant 3 (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213623
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001079821, NP_001073289
Synonyms AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; DFNA34; FCAS; FCAS1; FCU; KEFH; MWS; NALP3; PYPAF1
Predicted MW 118 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001073289
Locus ID 114548
Cytogenetics 1q44
Protein Families WB
Protein Pathways NOD-like receptor signaling pathway
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Other "NLRP3" proteins (8)
SKU Description Size Price
LC401526 Lyophilized NLRP3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC421550 Lyophilized NLRP3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426795 Lyophilized NLRP3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401526 Transient overexpression lysate of NLR family, pyrin domain containing 3 (NLRP3), transcript variant 1 (as WB positive control) 100 ug
$665.00
LY426795 Transient overexpression lysate of NLR family, pyrin domain containing 3 (NLRP3), transcript variant 4 (as WB positive control) 100 ug
$665.00
TP320952 Purified recombinant protein of Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 1, full length, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20 µg 20 ug
$867.00
TP750176 Purified recombinant protein of Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 3, full length, with C-terminal HIS tag, expressed in E.Coli, 50 ug 50 ug
$270.00
TP762656 Purified recombinant protein of Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 4, full length, with N-GST and C-His tag, expressed in E.coli, 50ug 50 ug
$270.00

file-alt Citations

Inflammasome-Independent Role of NLRP3 Mediates Mitochondrial Regulation in Renal Injury
Kim, Kim, Kim et al
Front Immunol (2018) 9, 2563 DOI: 10.3389/fimmu.2018.02563
AI Summary: OriGene’s NLRP3-pCMV6 vector was used for transient transfection to study the role of NLRP3 in mitochondrial regulation in renal tubular cells.
Snippet: Transient transfection of NLRP3-pCMV6 vector (ORIGENE, Rockville, MD) was conducted using the Lipofectamine 2,000 method. LC3-GFP vector was kindly provided by Dr. DH Cho (Kyung Hee University School of East-West Medical Science). The human renal proximal tubular epithelial cell line (HKC8) was obtained from Dr. L. …
FigureFigureFigure
NLRP3 expression in mesencephalic neurons and characterization of a rare NLRP3 polymorphism associated with decreased risk of Parkinson's disease
von Herrmann, Salas, Martinez et al
NPJ Parkinsons Dis (2018) 4, 24 DOI: 10.1038/s41531-018-0061-5
AI Summary: OriGene’s human NLRP3 gene clone (RC220952) was used as the template for site-directed mutagenesis to generate NLRP3 variants for functional studies in this paper.
Snippet: ssion inheritance model was used to evaluate the chi-squared test for linear trend of the variants. Site-directed mutagenesis DNA corresponding to the human NLRP3 gene (NM_004895) was purchased from Origene (clone RC220952, Rockville, MD). Site-directed mutagenesis to generate NLRP3 harboring the rs7525979 variant (NLRP3979) was performed using the Stratagene QuikChange Kit (Agilent Technologies,
FigureFigureFigure
Showing 1-2 of 2 papers.
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