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NLRP3 (NM_001079821) Human Recombinant Protein

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SKU
TP750176
Purified recombinant protein of Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 3, full length, with C-terminal HIS tag, expressed in E.Coli, 50 ug
Size:
50 ug
  $270.00
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Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
A DNA sequence encoding the full length of NLRP3
Tag C-HIS
Predicted MW 118KDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_001073289
Locus ID 114548
UniProt ID Q96P20
Cytogenetics 1q44
RefSeq Size 3845
RefSeq ORF 3108
Synonyms AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; DFNA34; FCAS; FCAS1; FCU; KEFH; MWS; NALP3; PYPAF1
Summary This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, and deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. provided by RefSeq, Aug 2020
Protein Categories Allergies and autoimmune diseases, Cytokines, Intracellular Proteins, Nervous system Diseases
Protein Families Druggable Genome
Protein Pathways NOD-like receptor signaling pathway
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Other "NLRP3" proteins (8)
SKU Description Size Price
LC401526 Lyophilized NLRP3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC421550 Lyophilized NLRP3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426795 Lyophilized NLRP3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401526 Transient overexpression lysate of NLR family, pyrin domain containing 3 (NLRP3), transcript variant 1 (as WB positive control) 100 ug
$665.00
LY421550 Transient overexpression lysate of NLR family, pyrin domain containing 3 (NLRP3), transcript variant 3 (as WB positive control) 100 ug
$665.00
LY426795 Transient overexpression lysate of NLR family, pyrin domain containing 3 (NLRP3), transcript variant 4 (as WB positive control) 100 ug
$665.00
TP320952 Purified recombinant protein of Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 1, full length, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20 µg 20 ug
$867.00
TP762656 Purified recombinant protein of Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 4, full length, with N-GST and C-His tag, expressed in E.coli, 50ug 50 ug
$270.00

file-alt Citations

In Silico and In Vitro Evaluation of Quercetin Metabolites Binding to Inflammatory Target Proteins
Yücer, Periasamy, Guthart et al
Pharmaceuticals (2026) 19 (5), 655-655 DOI: 10.3390/ph19050655
Cited by 1
AI Summary: OriGene’s recombinant NLRP3 protein (TP750176) was used as a key reagent in binding studies to evaluate interactions between quercetin metabolites and the inflammasome protein in this research.
Snippet: Recombinant NLRP3 (Origene, TP750176) was labeled with the Monolith Protein Labeling Kit RED-NHS 2nd Generation (Cat. No. M0-L011, NanoTemper Technologies, Munich, Germany)
Identification of anti-inflammatory and anti-cancer compounds targeting the NF-κB-NLRP3 inflammasome pathway from a phytochemical library of the Sideritis genus
Yücer, Schröder, Topçu et al
J Ethnopharmacol (2024) 338 (Pt 2), 119074 DOI: 10.1016/j.jep.2024.119074
Cited by 7
AI Summary: OriGene’s recombinant NLRP3 protein (TP750176) was used in microscale thermophoresis assays to experimentally confirm the binding of Sideritis-derived compounds to NLRP3, supporting the study’s investigation of anti-inflammatory and anti-cancer activities.
Snippet: The confirmation of the in silico binding results was performed with selected compounds using microscale thermophoresis (MST). Recombinant NLRP3 (Origene, TP750176) and NF-κB p100/p52 (Abcam, ab125611) were labeled with the Monolith Protein Labeling Kit RED-NHS 2nd Generation (Cat. No. M0-L011, NanoTemper Technologies, Munich,
Chloranthalactone B covalently binds to the NACHT domain of NLRP3 to attenuate NLRP3-driven inflammation
Tang, Zhao, Wang et al
Biochem Pharmacol (2024) 226, 116360 DOI: 10.1016/j.bcp.2024.116360
Cited by 9
AI Summary: OriGene contributed to this study by providing the recombinant His-NLRP3 protein, which was essential for investigating the binding interactions and inhibitory effects of Chloranthalactone B on NLRP3-driven inflammation.
Snippet: and MSU were purchased from Sigma (St.Louis, MO, USA). The recombinant His-NLRP3 protein was purchased from OriGene Technologies Inc (Rockville, MD, USA). Anti-NLRP3, anti-NEK7, and anti-IL-1β antibodies were obtained from Cell Signaling Technology (Beverly, MA). Anti-ASC and anti-caspase-1 antibodies were obtained from Abcam (London, UK)
Showing 1-3 of 3 papers.
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