OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

AP2 alpha (TFAP2A) (NM_001042425) Human Over-expression Lysate

Be the first to review this product
SKU
LY420895
Transient overexpression lysate of transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha) (TFAP2A), transcript variant 3 (as WB positive control)
  $665.00
In Stock*
Bulk/Customize

Frequently Bought Together (3)

TA313238 Rabbit polyclonal anti-AP-2 antibody
100 ul
$380.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "AP2 alpha or NM_001042425"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC220080
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001042425, NP_001035890
Synonyms AP-2; AP-2alpha; AP2TF; BOFS; TFAP2
Predicted MW 47.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001035890
Locus ID 7020
Cytogenetics 6p24.3
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "AP2 alpha" proteins (5)
SKU Description Size Price
PH304603 TFAP2A MS Standard C13 and N15-labeled recombinant protein (NP_001027451) 10 ug
$3,360.00
LC420895 Lyophilized TFAP2A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422292 Lyophilized TFAP2A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY422292 Transient overexpression lysate of transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha) (TFAP2A), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP304603 Recombinant protein of human transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha) (TFAP2A), transcript variant 2, 20 µg 20 ug
$867.00

file-alt Citations

A Functional Variant Associated with Atrial Fibrillation Regulates PITX2c Expression through TFAP2a
Ye, Tucker, Weng et al
Am. J. Hum. Genet. (2016) 99 (6), 1281-1291 DOI: 10.1016/j.ajhg.2016.10.001
AI Summary: OriGene’s purified TFAP2a protein was used in binding assays to investigate how the transcription factor interacts with regulatory DNA sequences near the PITX2c gene, helping to elucidate the molecular mechanism by which the AF-associated SNP rs2595104 affects gene expression.
Snippet: Pierce). In brief, the double-stranded probes (20 fmol) were incubated withpurified TFAP2a (Origene) at room temperature for 20 min in the presence of 100 mMTris (pH 7.5), 500 mM KCl, and 10 mM DTT in a 20 L reaction. ... Cite SaveSaving
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products