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AP2 alpha (TFAP2A) (NM_001042425) Human Over-expression Lysate

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SKU
LC420895
Lyophilized TFAP2A HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC220080
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001042425, NP_001035890
Synonyms AP-2; AP-2alpha; AP2TF; BOFS; TFAP2
Predicted MW 47.3 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001035890
Locus ID 7020
Cytogenetics 6p24.3
Protein Families WB
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Other "AP2 alpha" proteins (5)
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LC422292 Lyophilized TFAP2A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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file-alt Citations

A Functional Variant Associated with Atrial Fibrillation Regulates PITX2c Expression through TFAP2a
Ye, Tucker, Weng et al
Am. J. Hum. Genet. (2016) 99 (6), 1281-1291 DOI: 10.1016/j.ajhg.2016.10.001
AI Summary: OriGene’s purified TFAP2a protein was used in binding assays to investigate how the transcription factor interacts with regulatory DNA sequences near the PITX2c gene, helping to elucidate the molecular mechanism by which the AF-associated SNP rs2595104 affects gene expression.
Snippet: Pierce). In brief, the double-stranded probes (20 fmol) were incubated withpurified TFAP2a (Origene) at room temperature for 20 min in the presence of 100 mMTris (pH 7.5), 500 mM KCl, and 10 mM DTT in a 20 L reaction. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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