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BMP4 (NM_001202) Human Over-expression Lysate

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SKU
LY420075
Transient overexpression lysate of bone morphogenetic protein 4 (BMP4), transcript variant 1 (as WB positive control)
  • LY420075 is the same product as LY429051.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC204473
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001202, NP_001193
Synonyms BMP2B; BMP2B1; MCOPS6; OFC11; ZYME
Predicted MW 46.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001193
Locus ID 652
Cytogenetics 14q22.2
Protein Families WB
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer, TGF-beta signaling pathway
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Other "BMP4" proteins (9)
SKU Description Size Price
LC403337 Lyophilized BMP4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC408780 Lyophilized BMP4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC420075 Lyophilized BMP4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403337 Transient overexpression lysate of bone morphogenetic protein 4 (BMP4), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY408780 Transient overexpression lysate of bone morphogenetic protein 4 (BMP4), transcript variant 3 (as WB positive control) 100 ug
$436.00
TP750186 Purified recombinant protein of Human bone morphogenetic protein 4 (BMP4), transcript variant 1, Ser293-End, with N-terminal T7 tag, expressed in E.coli, 50ug 50 ug
$380.00
TP750195 Purified recombinant protein of Human bone morphogenetic protein 4 (BMP4), transcript variant 1, Ser293-End, tag free, expressed in E.coli, 50ug 50 ug
$380.00
TP761235 Purified recombinant protein of Human bone morphogenetic protein 4 (BMP4), transcript variant 11, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00
TP761239 Purified recombinant protein of Human bone morphogenetic protein 4 (BMP4), transcript variant 3, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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