BMP4 (NM_001202) Human Tagged ORF Clone

SKU
RC204473
BMP4 (Myc-DDK-tagged)-Human bone morphogenetic protein 4 (BMP4), transcript variant 1
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    Protein expression verified by Western blot, fully sequenced and in stock

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$686.00
In Stock*
Specifications
Product Data
Target Symbol BMP4
Synonyms BMP2B; BMP2B1; MCOPS6; OFC11; ZYME
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001202
ORF Size 1224 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001202.2, NP_001193.1
RefSeq Size 1957 bp
RefSeq ORF 1227 bp
Locus ID 652
UniProt ID P12644
Cytogenetics 14q22.2
Domains TGF-beta, TGFb_propeptide
Protein Families Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer, TGF-beta signaling pathway
MW 46.5 kDa
Summary This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers. [provided by RefSeq, Jul 2016]
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SKU Description Size Price
RC204473L1 Lenti ORF clone of Human bone morphogenetic protein 4 (BMP4), transcript variant 1, Myc-DDK-tagged 10 ug
$986.00
RC204473L2 Lenti ORF clone of Human bone morphogenetic protein 4 (BMP4), transcript variant 1, mGFP tagged 10 ug
$986.00
RC204473L3 Lenti ORF clone of Human bone morphogenetic protein 4 (BMP4), transcript variant 1, Myc-DDK-tagged 10 ug
$986.00
RC204473L4 Lenti ORF clone of Human bone morphogenetic protein 4 (BMP4), transcript variant 1, mGFP tagged 10 ug
$986.00
RG204473 BMP4 (tGFP-tagged) - Human bone morphogenetic protein 4 (BMP4), transcript variant 1 10 ug
$886.00
SC108985 BMP4 (untagged)-Human bone morphogenetic protein 4 (BMP4), transcript variant 1 10 ug
$457.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.