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HNRNPD (NM_002138) Human Over-expression Lysate

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SKU
LY419510
Transient overexpression lysate of heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD), transcript variant 3 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200660
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002138, NP_002129
Synonyms AUF1; AUF1A; hnRNPD0; HNRPD; P37
Predicted MW 32.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002129
Locus ID 3184
Cytogenetics 4q21.22
Protein Families WB
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Other "HNRNPD" proteins (16)
SKU Description Size Price
PH300660 HNRNPD MS Standard C13 and N15-labeled recombinant protein (NP_002129) 10 ug
$3,360.00
PH301796 HNRNPD MS Standard C13 and N15-labeled recombinant protein (NP_112738) 10 ug
$3,360.00
PH320809 HNRNPD MS Standard C13 and N15-labeled recombinant protein (NP_001003810) 10 ug
$3,360.00
PH322094 HNRNPD MS Standard C13 and N15-labeled recombinant protein (NP_112737) 10 ug
$3,360.00
LC410547 Lyophilized HNRNPD HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC410548 Lyophilized HNRNPD HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC419510 Lyophilized HNRNPD HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424021 Lyophilized HNRNPD HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY410547 Transient overexpression lysate of heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY410548 Transient overexpression lysate of heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY424021 Transient overexpression lysate of heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD), transcript variant 4 (as WB positive control) 100 ug
$436.00
TP300660 Recombinant protein of human heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD), transcript variant 3, 20 µg 20 ug
$737.00
TP301796 Recombinant protein of human heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD), transcript variant 1, 20 µg 20 ug
$737.00
TP320809 Recombinant protein of human heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD), transcript variant 4, 20 µg 20 ug
$737.00
TP322094 Purified recombinant protein of Homo sapiens heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD), transcript variant 2, 20 µg 20 ug
$737.00
TP760280 Recombinant protein of human heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD), transcript variant 3, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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