MSI1 (NM_002442) Human Over-expression Lysate
SKU
LY419331
Transient overexpression lysate of musashi homolog 1 (Drosophila) (MSI1) (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC215992 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_002442, NP_002433 |
| Predicted MW | 38.9 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_002433 |
| Locus ID | 4440 |
| Cytogenetics | 12q24.31 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
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Resources
| Recombinant Protein Resources |
Other "MSI1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH315992 | MSI1 MS Standard C13 and N15-labeled recombinant protein (NP_002433) | 10 ug |
$3,360.00
|
|
| LC419331 | Lyophilized MSI1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP315992 | Recombinant protein of human musashi homolog 1 (Drosophila) (MSI1), 20 µg | 20 ug |
$737.00
|
|
Citations
Musashi expression in β-cells coordinates insulin expression, apoptosis and proliferation in response to endoplasmic reticulum stress in diabetes
Szabat, Kalynyak, Lim et al
Cell Death Dis (2011) 2, e232 DOI: 10.1038/cddis.2011.119
Szabat, Kalynyak, Lim et al
Cell Death Dis (2011) 2, e232 DOI: 10.1038/cddis.2011.119
Cited by 61
AI Summary: OriGene contributed to this study by providing RFP- and GFP-bearing shRNA constructs targeting Msi1 and Msi2, as well as human MSI1-GFP and GFP control overexpression plasmids, which were used to manipulate Musashi gene expression in pancreatic β-cells.
Snippet: Materials and Methods. Reagents. Respective RFP- and GFP-bearing shRNA constructs targeted to Msi1 and Msi2 were purchased from OriGene (Rockville, MD, USA). Overexpression constructs for human MSI1-GFP and GFP control plasmids were purchased from OriGene.
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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