OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

MSI1 (NM_002442) Human Over-expression Lysate

Be the first to review this product
SKU
LC419331
Lyophilized MSI1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

TA502198 MSI1 mouse monoclonal antibody, clone OTI1G8 (formerly 1G8) 100 ul
$447.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "MSI1 or NM_002442"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC215992
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002442, NP_002433
Predicted MW 38.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002433
Locus ID 4440
Cytogenetics 12q24.31
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "MSI1" proteins (3)
SKU Description Size Price
PH315992 MSI1 MS Standard C13 and N15-labeled recombinant protein (NP_002433) 10 ug
$3,360.00
LY419331 Transient overexpression lysate of musashi homolog 1 (Drosophila) (MSI1) (as WB positive control) 100 ug
$436.00
TP315992 Recombinant protein of human musashi homolog 1 (Drosophila) (MSI1), 20 µg 20 ug
$737.00

file-alt Citations

Musashi expression in β-cells coordinates insulin expression, apoptosis and proliferation in response to endoplasmic reticulum stress in diabetes
Szabat, Kalynyak, Lim et al
Cell Death Dis (2011) 2, e232 DOI: 10.1038/cddis.2011.119
Cited by 61
AI Summary: OriGene contributed to this study by providing RFP- and GFP-bearing shRNA constructs targeting Msi1 and Msi2, as well as human MSI1-GFP and GFP control overexpression plasmids, which were used to manipulate Musashi gene expression in pancreatic β-cells.
Snippet: Materials and Methods. Reagents. Respective RFP- and GFP-bearing shRNA constructs targeted to Msi1 and Msi2 were purchased from OriGene (Rockville, MD, USA). Overexpression constructs for human MSI1-GFP and GFP control plasmids were purchased from OriGene.
FigureFigureFigure
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products