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S100A5 (NM_002962) Human Over-expression Lysate

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SKU
LY418992
Transient overexpression lysate of S100 calcium binding protein A5 (S100A5) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210891
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002962, NP_002953
Synonyms S100D
Predicted MW 12.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002953
Locus ID 6276
Cytogenetics 1q21.3
Protein Families WB
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Other "S100A5" proteins (3)
SKU Description Size Price
PH310891 S100A5 MS Standard C13 and N15-labeled recombinant protein (NP_002953) 10 ug
$3,360.00
LC418992 Lyophilized S100A5 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP310891 Recombinant protein of human S100 calcium binding protein A5 (S100A5), 20 µg 20 ug
$867.00

file-alt Citations

Quantitative Proteomics Reveal an Altered Pattern of Protein Expression in Brain Tissue from Mice Lacking GPR37 and GPR37L1
Nguyen, Dammer, Owino et al
J. Proteome Res. (2020) 19 (2), 744-755 DOI: 10.1021/acs.jproteome.9b00622
Cited by 14
AI Summary: OriGene products—specifically human S100A5-myc-DDK, human S100A4-myc-DDK, and human S100A6-myc-DDK—were used in this study to generate cell lysates for validating S100A5 antibody specificity in HEK293T cells.
Snippet: human S100A5-myc-DDK (Origene), human S100A4-myc-DDK (Origene), and human S100A6- myc-DDK (Origene). Cell lysate preparation S100A5 antibody validation Lysates from HEK293T cells that were transfected with either S100A4-mycDDK (Origene);- human S100A5-myc-DDK (Origene), human S100A4-myc-DDK (Origene), and human S100A6- myc-DDK (Origene). Cell lysate preparation S100A5 antibody validation Lysates from HEK293T cells that were transfected with either S100A4-mycDDK (Origene)
Showing 1-1 of 1 papers.
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