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S100A5 (NM_002962) Human Over-expression Lysate

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SKU
LC418992
Lyophilized S100A5 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210891
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002962, NP_002953
Synonyms S100D
Predicted MW 12.8 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002953
Locus ID 6276
Cytogenetics 1q21.3
Protein Families WB
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Other "S100A5" proteins (3)
SKU Description Size Price
PH310891 S100A5 MS Standard C13 and N15-labeled recombinant protein (NP_002953) 10 ug
$3,360.00
LY418992 Transient overexpression lysate of S100 calcium binding protein A5 (S100A5) (as WB positive control) 100 ug
$436.00
TP310891 Recombinant protein of human S100 calcium binding protein A5 (S100A5), 20 µg 20 ug
$867.00

file-alt Citations

Quantitative Proteomics Reveal an Altered Pattern of Protein Expression in Brain Tissue from Mice Lacking GPR37 and GPR37L1
Nguyen, Dammer, Owino et al
J. Proteome Res. (2020) 19 (2), 744-755 DOI: 10.1021/acs.jproteome.9b00622
Cited by 14
AI Summary: OriGene products—specifically human S100A5-myc-DDK, human S100A4-myc-DDK, and human S100A6-myc-DDK—were used in this study to generate cell lysates for validating S100A5 antibody specificity in HEK293T cells.
Snippet: human S100A5-myc-DDK (Origene), human S100A4-myc-DDK (Origene), and human S100A6- myc-DDK (Origene). Cell lysate preparation S100A5 antibody validation Lysates from HEK293T cells that were transfected with either S100A4-mycDDK (Origene);- human S100A5-myc-DDK (Origene), human S100A4-myc-DDK (Origene), and human S100A6- myc-DDK (Origene). Cell lysate preparation S100A5 antibody validation Lysates from HEK293T cells that were transfected with either S100A4-mycDDK (Origene)
Showing 1-1 of 1 papers.
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