S100A5 (NM_002962) Human Tagged ORF Clone
$150.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | S100A5 |
| Synonyms | S100D |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002962 |
| ORF Size | 330 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq Size | 710 bp |
| RefSeq ORF | 279 bp |
| Locus ID | 6276 |
| UniProt ID | P33763 |
| Cytogenetics | 1q21.3 |
| MW | 12.8 kDa |
| Summary | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002962" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210891L1 | Lenti ORF clone of Human S100 calcium binding protein A5 (S100A5), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC210891L2 | Lenti ORF clone of Human S100 calcium binding protein A5 (S100A5), mGFP tagged | 10 ug |
$450.00
|
|
| RC210891L3 | Lenti ORF clone of Human S100 calcium binding protein A5 (S100A5), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC210891L4 | Lenti ORF clone of Human S100 calcium binding protein A5 (S100A5), mGFP tagged | 10 ug |
$450.00
|
|
| RG210891 | S100A5 (tGFP-tagged) - Human S100 calcium binding protein A5 (S100A5) | 10 ug |
$489.00
|
|
| SC303255 | S100A5 (untagged)-Human S100 calcium binding protein A5 (S100A5) | 10 ug |
$165.00
|
|
Citations
Quantitative Proteomics Reveal an Altered Pattern of Protein Expression in Brain Tissue from Mice Lacking GPR37 and GPR37L1
Nguyen, Dammer, Owino et al
J. Proteome Res. (2020) 19 (2), 744-755 DOI: 10.1021/acs.jproteome.9b00622
Nguyen, Dammer, Owino et al
J. Proteome Res. (2020) 19 (2), 744-755 DOI: 10.1021/acs.jproteome.9b00622
Cited by 14
AI Summary: OriGene products—specifically human S100A5-myc-DDK, human S100A4-myc-DDK, and human S100A6-myc-DDK—were used in this study to generate cell lysates for validating S100A5 antibody specificity in HEK293T cells.
Snippet: human S100A5-myc-DDK (Origene), human S100A4-myc-DDK (Origene), and human S100A6- myc-DDK (Origene). Cell lysate preparation S100A5 antibody validation Lysates from HEK293T cells that were transfected with either S100A4-mycDDK (Origene);- human S100A5-myc-DDK (Origene), human S100A4-myc-DDK (Origene), and human S100A6- myc-DDK (Origene). Cell lysate preparation S100A5 antibody validation Lysates from HEK293T cells that were transfected with either S100A4-mycDDK (Origene)
Showing 1-1 of 1 papers.
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