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SOX4 (NM_003107) Human Over-expression Lysate

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SKU
LY418894
Transient overexpression lysate of SRY (sex determining region Y)-box 4 (SOX4) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209139
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003107, NP_003098
Synonyms CSS10; EVI16
Predicted MW 47.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_003098
Locus ID 6659
Cytogenetics 6p22.3
Protein Families WB
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Other "SOX4" proteins (3)
SKU Description Size Price
PH309139 SOX4 MS Standard C13 and N15-labeled recombinant protein (NP_003098) 10 ug
$3,360.00
LC418894 Lyophilized SOX4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP309139 Recombinant protein of human SRY (sex determining region Y)-box 4 (SOX4), 20 µg 20 ug
$737.00

file-alt Citations

MicroRNA-338-3p targets SOX4 and inhibits cell proliferation and invasion of renal cell carcinoma
Tong, Meng, Wang et al
Exp Ther Med (2017) 14 (5), 5200-5206 DOI: 10.3892/etm.2017.5169
Cited by 25
AI Summary: OriGene’s SOX4 overexpression plasmid was used in this study to investigate the role of SOX4 in renal cell carcinoma cells and to assess how SOX4 overexpression could counteract the tumor-suppressive effects of miR-338-3p.
Snippet: A SOX4 overexpression plasmid was purchased from OriGene Technologies, Inc., (Beijing, China). miR-338-3p (100 nM), miR-NC (100 nM) or the SOX4 plasmid (100 ng) were transfected into RCC cells using Lipofectamine 2000 (Invitrogen;Thermo Fisher Scientific, Inc.), according to the manufacturer's instructions
Down-regulated SOX4 expression suppresses cell proliferation, metastasis and induces apoptosis in Xuanwei female lung cancer patients
Zhou, Wang, Huang et al
J. Cell. Biochem. (2015)
Cited by 37
AI Summary: OriGene's pGFP-V-RS retroviral vector was used to deliver SOX4-targeting shRNA into lung cancer cells, enabling the authors to investigate the effects of SOX4 knockdown on cell proliferation, metastasis, and apoptosis.
Snippet: Cell transfection SOX4 small hairpin RNA (shRNA) was designed and cloned into the pGFP-V-RS retroviral vector (Origene, Maryland, USA). The pGFP-V-RS-SOX4 shRNA sequence was AGCAAACCGCACGCCAAGCTCATCCTGGC, and the negative control shRNA ... Related articles All 3 versions Cite SaveSaving
Showing 1-2 of 2 papers.
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