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p63 (TP63) (NM_003722) Human Over-expression Lysate

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SKU
LY418475
Transient overexpression lysate of tumor protein p63 (TP63), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208013
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003722, NP_003713
Synonyms AIS; B(p51A); B(p51B); EEC3; KET; LMS; NBP; OFC8; p40; p51; p53CP; p63; p73H; p73L; RHS; SHFM4; TP53CP; TP53L; TP73L
Predicted MW 76.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_003713
Locus ID 8626
Cytogenetics 3q28
Protein Families WB
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Other "p63" proteins (12)
SKU Description Size Price
PH308013 TP63 MS Standard C13 and N15-labeled recombinant protein (NP_003713) 10 ug
$3,360.00
LC418475 Lyophilized TP63 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426514 Lyophilized TP63 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426515 Lyophilized TP63 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426516 Lyophilized TP63 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426518 Lyophilized TP63 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY426514 Transient overexpression lysate of tumor protein p63 (TP63), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY426515 Transient overexpression lysate of tumor protein p63 (TP63), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY426516 Transient overexpression lysate of tumor protein p63 (TP63), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY426518 Transient overexpression lysate of tumor protein p63 (TP63), transcript variant 6 (as WB positive control) 100 ug
$436.00
TP308013 Recombinant protein of human tumor protein p63 (TP63), transcript variant 1, 20 µg 20 ug
$737.00
TP710041 Recombinant protein of human tumor protein p63 (TP63), transcript variant 1, with C-terminal DDK tag,expressed in sf9 cells. 20 ug
$540.00

file-alt Citations

An Immunohistochemical Analysis of a Newly Developed, Mouse Monoclonal p40 (BC28) in Lung, Bladder, Skin, Breast, Prostate, and Head and Neck Cancers
Tacha, Bremer, Haas et al
Arch. Pathol. Lab. Med. (2014)
AI Summary: OriGene’s transactivated p63-transfected cell lysates were used for Western blotting to characterize the specificity of the newly developed MMp40 (BC28) monoclonal antibody in this study.
Snippet: Cross-reactivity Tested by Western Blotting The purified monoclonal antibody MMp40 (BC28) was charac- terized by Western blotting (Figure 1). Full-length p40 and transactivated p63-transfected cell lysates (OriGene, Rockville, Maryland) were subjected to protein ... Cite SaveSaving
Showing 1-1 of 1 papers.
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