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ROR2 (NM_004560) Human Over-expression Lysate

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SKU
LY417906
Transient overexpression lysate of receptor tyrosine kinase-like orphan receptor 2 (ROR2) (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC215640
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004560, NP_004551
Synonyms BDB; BDB1; NTRKR2
Predicted MW 104.74 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004551
Locus ID 4920
Cytogenetics 9q22.31
Protein Families WB
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Other "ROR2" proteins (6)
SKU Description Size Price
PH315640 ROR2 MS Standard C13 and N15-labeled recombinant protein (NP_004551) 10 ug
$3,360.00
LC417906 Lyophilized ROR2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP315640 Recombinant protein of human receptor tyrosine kinase-like orphan receptor 2 (ROR2), 20 µg 20 ug
$737.00
TP700160 Purified recombinant protein of Human receptor tyrosine kinase-like orphan receptor 2 (ROR2), with C-terminal DDK/His tag, expressed in human cells 20 ug
$900.00
TP710168 Purified protein of Human receptor tyrosine kinase-like orphan receptor 2 (ROR2), residues 34-403aa, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
$540.00
TP762680 Purified recombinant protein of Human receptor tyrosine kinase-like orphan receptor 2 (ROR2), 34Glu-403Gly, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$270.00

file-alt Citations

Ror2, a developmentally regulated kinase, promotes tumor growth potential in renal cell carcinoma
Wright, Brannon, Gordan et al
Oncogene (2009) 28 (27), 2513-23 DOI: 10.1038/onc.2009.116
Cited by 116
AI Summary: In this study, OriGene's Ror2 shRNA (OriGene, Cat# TF314908) was used to knock down Ror2 expression in renal cell carcinoma cells, enabling the authors to investigate Ror2's role in regulating tumor growth, cell migration, and extracellular matrix remodeling.
FigureFigureFigure
Showing 1-1 of 1 papers.
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