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BUB3 (NM_004725) Human Over-expression Lysate

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SKU
LY417795
Transient overexpression lysate of budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3), transcript variant 1 (as WB positive control)
  • LY417795 is the same product as LY429219.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200376
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004725, NP_004716
Synonyms BUB3L; hBUB3
Predicted MW 37 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004716
Locus ID 9184
Cytogenetics 10q26.13
Protein Families WB
Protein Pathways Cell cycle
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Other "BUB3" proteins (7)
SKU Description Size Price
PH320961 BUB3 MS Standard C13 and N15-labeled recombinant protein (NP_001007794) 10 ug
$3,360.00
LC400384 Lyophilized BUB3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC417795 Lyophilized BUB3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC429219 Lyophilized BUB3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400384 Transient overexpression lysate of budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY429219 Transient overexpression lysate of budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP320961 Recombinant protein of human budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3), transcript variant 2, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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