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CRKL (NM_005207) Human Over-expression Lysate

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SKU
LY417444
Transient overexpression lysate of v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL) (as WB positive control)
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208129
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005207, NP_005198
Predicted MW 33.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005198
Locus ID 1399
Cytogenetics 22q11.21
Protein Families WB
Protein Pathways Chemokine signaling pathway, Chronic myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, Pathways in cancer, Regulation of actin cytoskeleton, Renal cell carcinoma
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Other "CRKL" proteins (3)
SKU Description Size Price
PH308129 CRKL MS Standard C13 and N15-labeled recombinant protein (NP_005198) 10 ug
$3,360.00
LC417444 Lyophilized CRKL HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP308129 Recombinant protein of human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL), 20 µg 20 ug
$867.00

file-alt Citations

CRKL protein overexpression enhances cell proliferation and invasion in pancreatic cancer
Fu, Dong, Xie et al
Tumour Biol. (2014)
Cited by 18
AI Summary: OriGene contributed to this study by providing the CRKL plasmid, which was used to overexpress CRKL in pancreatic cancer cell lines to investigate its role in cell proliferation and invasion.
Snippet: Cells were grown on sterile tissue culture dishes and were passaged every 2 days using 0.25 % trypsin (Invitrogen). The plasmid of CRKL was purchased from OriGene. Plas- mid was transfected into cells using Attractene Transfection Reagent (Qiagen, Hilden, Germany ... Cite SaveSaving
Clinical significance and biological roles of CRKL in human bladder carcinoma
Han, Luan, Xu et al
Tumour Biol. (2013)
Cited by 11
AI Summary: OriGene contributed to this study by providing the CRKL plasmid, which was used to overexpress CRKL in bladder cancer cell lines to investigate its role in cell proliferation and invasion.
Snippet: Cells were grown on sterilized culture dishes and were passaged every 2 days with 0.25 % trypsin (Invitrogen). The plasmid of CRKL was purchased from Origene. Plas- mid was transfected into cells using Attractene Transfection (Qiagen, Hilden, Germany). ... Cite SaveSaving
Showing 1-2 of 2 papers.
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