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CRKL (NM_005207) Human Over-expression Lysate

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SKU
LC417444
Lyophilized CRKL HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208129
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005207, NP_005198
Predicted MW 33.6 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_005198
Locus ID 1399
Cytogenetics 22q11.21
Protein Families WB
Protein Pathways Chemokine signaling pathway, Chronic myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, Pathways in cancer, Regulation of actin cytoskeleton, Renal cell carcinoma
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Other "CRKL" proteins (3)
SKU Description Size Price
PH308129 CRKL MS Standard C13 and N15-labeled recombinant protein (NP_005198) 10 ug
$3,360.00
LY417444 Transient overexpression lysate of v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL) (as WB positive control) 100 ug
$436.00
TP308129 Recombinant protein of human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL), 20 µg 20 ug
$867.00

file-alt Citations

CRKL protein overexpression enhances cell proliferation and invasion in pancreatic cancer
Fu, Dong, Xie et al
Tumour Biol. (2014)
Cited by 18
AI Summary: OriGene contributed to this study by providing the CRKL plasmid, which was used to overexpress CRKL in pancreatic cancer cell lines to investigate its role in cell proliferation and invasion.
Snippet: Cells were grown on sterile tissue culture dishes and were passaged every 2 days using 0.25 % trypsin (Invitrogen). The plasmid of CRKL was purchased from OriGene. Plas- mid was transfected into cells using Attractene Transfection Reagent (Qiagen, Hilden, Germany ... Cite SaveSaving
Clinical significance and biological roles of CRKL in human bladder carcinoma
Han, Luan, Xu et al
Tumour Biol. (2013)
Cited by 11
AI Summary: OriGene contributed to this study by providing the CRKL plasmid, which was used to overexpress CRKL in bladder cancer cell lines to investigate its role in cell proliferation and invasion.
Snippet: Cells were grown on sterilized culture dishes and were passaged every 2 days with 0.25 % trypsin (Invitrogen). The plasmid of CRKL was purchased from Origene. Plas- mid was transfected into cells using Attractene Transfection (Qiagen, Hilden, Germany). ... Cite SaveSaving
Showing 1-2 of 2 papers.
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