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Eph receptor A3 (EPHA3) (NM_005233) Human Over-expression Lysate

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SKU
LY417428
Transient overexpression lysate of EPH receptor A3 (EPHA3), transcript variant 1 (as WB positive control)
  $436.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209191
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005233, NP_005224
Synonyms EK4; ETK; ETK1; HEK; HEK4; TYRO4
Predicted MW 110 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005224
Locus ID 2042
Cytogenetics 3p11.1
Protein Families WB
Protein Pathways Axon guidance
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Other "Eph receptor A3" proteins (7)
SKU Description Size Price
PH309191 EPHA3 MS Standard C13 and N15-labeled recombinant protein (NP_005224) 10 ug
$3,360.00
LC417428 Lyophilized EPHA3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP309191 Recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 1, 20 µg 20 ug
$867.00
TP700139 Purified recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 1, with C-terminal DDK/His tag, expressed in human cells, 20 µg 20 ug
$900.00
TP700140 Purified recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 2, with C-terminal DDK/His tag, expressed in human cells, 20 µg 20 ug
$900.00
TP710104 Recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 1, residues 21-541aa, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
$540.00
TP723965 Recombinant human EPHA3 protein with C-terminal mouse Fc and 6×His tag 100 ug
$795.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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