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Eph receptor A3 (EPHA3) (NM_005233) Human Over-expression Lysate

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SKU
LC417428
Lyophilized EPHA3 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209191
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005233, NP_005224
Synonyms EK4; ETK; ETK1; HEK; HEK4; TYRO4
Predicted MW 110 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_005224
Locus ID 2042
Cytogenetics 3p11.1
Protein Families WB
Protein Pathways Axon guidance
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Other "Eph receptor A3" proteins (7)
SKU Description Size Price
PH309191 EPHA3 MS Standard C13 and N15-labeled recombinant protein (NP_005224) 10 ug
$3,360.00
LY417428 Transient overexpression lysate of EPH receptor A3 (EPHA3), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP309191 Recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 1, 20 µg 20 ug
$867.00
TP700139 Purified recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 1, with C-terminal DDK/His tag, expressed in human cells, 20 µg 20 ug
$900.00
TP700140 Purified recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 2, with C-terminal DDK/His tag, expressed in human cells, 20 µg 20 ug
$900.00
TP710104 Recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 1, residues 21-541aa, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
$540.00
TP723965 Recombinant human EPHA3 protein with C-terminal mouse Fc and 6×His tag 100 ug
$795.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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