Eph receptor A3 (EPHA3) Human Recombinant Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Mouse Fc and 6×His Tag |
| Predicted MW | The protein has a predicted molecular mass of 85.9 kDa after removal of the signal peptide. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Buffer | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization |
| Reconstitution Method | 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube. 2. Calculate the volume for reconstitution (in µL) using the formula: Quantity (mg)/Concentration (mg/mL)x1000 3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL) 4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample. |
| Preparation | Affinity purification |
| Recommended Dilution | ELISA |
| Storage | Store the lyophilized protein at -20°C. After reconstitution, store the protein at -80°C for 12 months. Avoid repeated freezing and thawing. |
| Stability | 12 months from date of despatch |
| Shipping | Ambient |
| Reference Data | |
| Locus ID | 2042 |
| UniProt ID | P29320 |
| Synonyms | TYRO4;HEK4;ETK1;ETK;EK4;HEK |
| Summary | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. |
| Protein Categories | Enzyme: Kinases, Intracellular Proteins, Membrane Proteins |
| Protein Families | ELISA |
| Protein Pathways | Axon guidance |
Reviews
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Resources
| Recombinant Protein Resources |
Other "Eph receptor A3" proteins (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH309191 | EPHA3 MS Standard C13 and N15-labeled recombinant protein (NP_005224) | 10 ug |
$3,360.00
|
|
| LC417428 | Lyophilized EPHA3 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY417428 | Transient overexpression lysate of EPH receptor A3 (EPHA3), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| TP309191 | Recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 1, 20 µg | 20 ug |
$867.00
|
|
| TP700139 | Purified recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 1, with C-terminal DDK/His tag, expressed in human cells, 20 µg | 20 ug |
$900.00
|
|
| TP700140 | Purified recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 2, with C-terminal DDK/His tag, expressed in human cells, 20 µg | 20 ug |
$900.00
|
|
| TP710104 | Recombinant protein of human EPH receptor A3 (EPHA3), transcript variant 1, residues 21-541aa, with C-terminal DDK tag, expressed in sf9, 20ug | 20 ug |
$540.00
|
|
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