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DDX58 (NM_014314) Human Over-expression Lysate

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SKU
LY415370
Transient overexpression lysate of DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58) (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217615
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_014314, NP_055129
Synonyms RIG-I; RIG1; RIGI; RLR-1; SGMRT2
Predicted MW 106.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_055129
Locus ID 23586
Cytogenetics 9p21.1
Protein Families WB
Protein Pathways Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway
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Other "DDX58" proteins (3)
SKU Description Size Price
PH317615 DDX58 MS Standard C13 and N15-labeled recombinant protein (NP_055129) 10 ug
$3,360.00
LC415370 Lyophilized DDX58 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP317615 Recombinant protein of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), 20 µg 20 ug
$737.00

file-alt Citations

Type-I interferons induce lung protease responses following respiratory syncytial virus infection via RIG-I-like receptors
Foronjy, Taggart, Dabo et al
Mucosal Immunol (2015) 8 (1), 161-75 DOI: 10.1038/mi.2014.54
Cited by 42
AI Summary: In this study, OriGene’s RIG-I (NM_014314) Human cDNA ORF Clone was used to overexpress RIG-I in lung epithelial cells, enabling the authors to demonstrate that RIG-I activation induces protease expression following RSV infection.
FigureFigureFigure
Showing 1-1 of 1 papers.
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