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DDX58 (NM_014314) Human Over-expression Lysate

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SKU
LC415370
Lyophilized DDX58 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217615
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_014314, NP_055129
Synonyms RIG-I; RIG1; RIGI; RLR-1; SGMRT2
Predicted MW 106.4 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_055129
Locus ID 23586
Cytogenetics 9p21.1
Protein Families WB
Protein Pathways Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway
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Other "DDX58" proteins (3)
SKU Description Size Price
PH317615 DDX58 MS Standard C13 and N15-labeled recombinant protein (NP_055129) 10 ug
$3,360.00
LY415370 Transient overexpression lysate of DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58) (as WB positive control) 100 ug
$665.00
TP317615 Recombinant protein of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), 20 µg 20 ug
$737.00

file-alt Citations

Type-I interferons induce lung protease responses following respiratory syncytial virus infection via RIG-I-like receptors
Foronjy, Taggart, Dabo et al
Mucosal Immunol (2015) 8 (1), 161-75 DOI: 10.1038/mi.2014.54
Cited by 42
AI Summary: In this study, OriGene’s RIG-I (NM_014314) Human cDNA ORF Clone was used to overexpress RIG-I in lung epithelial cells, enabling the authors to demonstrate that RIG-I activation induces protease expression following RSV infection.
FigureFigureFigure
Showing 1-1 of 1 papers.
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