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Staufen (STAU1) (NM_017454) Human Over-expression Lysate

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SKU
LY413758
Transient overexpression lysate of staufen, RNA binding protein, homolog 1 (Drosophila) (STAU1), transcript variant T1 (as WB positive control)
  • LY413758 is the same product as LY429537.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $665.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC215637
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_017454, NP_059348
Synonyms PPP1R150; STAU
Predicted MW 54.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_059348
Locus ID 6780
Cytogenetics 20q13.13
Protein Families WB
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Other "Staufen" proteins (13)
SKU Description Size Price
PH308387 STAU1 MS Standard C13 and N15-labeled recombinant protein (NP_059347) 10 ug
$3,360.00
LC413756 Lyophilized STAU1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC413757 Lyophilized STAU1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC413758 Lyophilized STAU1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC417883 Lyophilized STAU1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC421948 Lyophilized STAU1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY413756 Transient overexpression lysate of staufen, RNA binding protein, homolog 1 (Drosophila) (STAU1), transcript variant T2 (as WB positive control) 100 ug
$665.00
LY413757 Transient overexpression lysate of staufen, RNA binding protein, homolog 1 (Drosophila) (STAU1), transcript variant T3 (as WB positive control) 100 ug
$436.00
LY417883 Transient overexpression lysate of staufen, RNA binding protein, homolog 1 (Drosophila) (STAU1), transcript variant T4 (as WB positive control) 100 ug
$436.00
LY421948 Transient overexpression lysate of staufen, RNA binding protein, homolog 1 (Drosophila) (STAU1), transcript variant T5 (as WB positive control) 100 ug
$665.00
TP308387 Recombinant protein of human staufen, RNA binding protein, homolog 1 (Drosophila) (STAU1), transcript variant T3, 20 µg 20 ug
$867.00
TP760787 Purified recombinant protein of Human staufen, RNA binding protein, homolog 1 (Drosophila) (STAU1), transcript variant T2, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00
TP761139 Purified recombinant protein of Human staufen, RNA binding protein, homolog 1 (Drosophila) (STAU1), transcript variant T4, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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