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HDAC8 (NM_018486) Human Over-expression Lysate

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SKU
LY413013
Transient overexpression lysate of histone deacetylase 8 (HDAC8), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208390
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_018486, NP_060956
Synonyms CDA07; CDLS5; HD8; HDACL1; KDAC8; MRXS6; RPD3; WTS
Predicted MW 41.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_060956
Locus ID 55869
Cytogenetics Xq13.1
Protein Families WB
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Other "HDAC8" proteins (4)
SKU Description Size Price
LC413013 Lyophilized HDAC8 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC432831 Lyophilized HDAC8 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY432831 Transient overexpression lysate of histone deacetylase 8 (HDAC8), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP760640 Purified recombinant protein of Human histone deacetylase 8 (HDAC8), transcript variant 1, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

Isoform-specific characterization of class I histone deacetylases and their therapeutic modulation in pulmonary hypertension
Chelladurai, Dabral, Basineni et al
Sci Rep (2020) 10 (1), 12864 DOI: 10.1038/s41598-020-69737-x
Cited by 42
AI Summary: OriGene Technologies contributed to this study by providing the HDAC8 overexpression plasmid, which was used to transiently transfect cells for functional analysis of class I HDAC isoforms in pulmonary hypertension.
Snippet: 4 or HEK293 were transiently transfected with specific plasmids overexpressing HDAC1 (Sino Biological Inc.), HDAC2 (received as a gift from Prof. Edward Seto, George Washington Cancer Center), HDAC8 (OriGene Technologies Inc.) or LEF1 (Sino Biological Inc.) using either Amaxa Basic Nucleofecto Kit for Primary Mammalian Fibroblasts or TurboFect Transfection Reagent (Thermo Scientific), according to
FigureFigureFigure
Showing 1-1 of 1 papers.
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