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HDAC8 (NM_018486) Human Over-expression Lysate

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SKU
LC413013
Lyophilized HDAC8 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208390
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_018486, NP_060956
Synonyms CDA07; CDLS5; HD8; HDACL1; KDAC8; MRXS6; RPD3; WTS
Predicted MW 41.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_060956
Locus ID 55869
Cytogenetics Xq13.1
Protein Families WB
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Other "HDAC8" proteins (4)
SKU Description Size Price
LC432831 Lyophilized HDAC8 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
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LY432831 Transient overexpression lysate of histone deacetylase 8 (HDAC8), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP760640 Purified recombinant protein of Human histone deacetylase 8 (HDAC8), transcript variant 1, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
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file-alt Citations

Isoform-specific characterization of class I histone deacetylases and their therapeutic modulation in pulmonary hypertension
Chelladurai, Dabral, Basineni et al
Sci Rep (2020) 10 (1), 12864 DOI: 10.1038/s41598-020-69737-x
Cited by 42
AI Summary: OriGene Technologies contributed to this study by providing the HDAC8 overexpression plasmid, which was used to transiently transfect cells for functional analysis of class I HDAC isoforms in pulmonary hypertension.
Snippet: 4 or HEK293 were transiently transfected with specific plasmids overexpressing HDAC1 (Sino Biological Inc.), HDAC2 (received as a gift from Prof. Edward Seto, George Washington Cancer Center), HDAC8 (OriGene Technologies Inc.) or LEF1 (Sino Biological Inc.) using either Amaxa Basic Nucleofecto Kit for Primary Mammalian Fibroblasts or TurboFect Transfection Reagent (Thermo Scientific), according to
FigureFigureFigure
Showing 1-1 of 1 papers.
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