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CEACAM19 (NM_020219) Human Over-expression Lysate

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SKU
LY412583
Transient overexpression lysate of carcinoembryonic antigen-related cell adhesion molecule 19 (CEACAM19), transcript variant 2 (as WB positive control)
  $436.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC215862
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_020219, NP_064604
Synonyms CEACM19; CEAL1
Predicted MW 32.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_064604
Locus ID 56971
Cytogenetics 19q13.31
Protein Families WB
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Other "CEACAM19" proteins (7)
SKU Description Size Price
PH315862 CEACAM19 MS Standard C13 and N15-labeled recombinant protein (NP_064604) 10 ug
$3,360.00
PH325416 CEACAM19 MS Standard C13 and N15-labeled recombinant protein (NP_001121365) 10 ug
$3,360.00
LC412583 Lyophilized CEACAM19 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426880 Lyophilized CEACAM19 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY426880 Transient overexpression lysate of carcinoembryonic antigen-related cell adhesion molecule 19 (CEACAM19), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP315862 Recombinant protein of human carcinoembryonic antigen-related cell adhesion molecule 19 (CEACAM19), transcript variant 2, 20 µg 20 ug
$867.00
TP325416 Recombinant protein of human carcinoembryonic antigen-related cell adhesion molecule 19 (CEACAM19), transcript variant 1, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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