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PINK1 (NM_032409) Human Over-expression Lysate

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SKU
LY410150
Transient overexpression lysate of PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein (as WB positive control)
  $436.00
4 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206970
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_032409, NP_115785
Synonyms BRPK; PARK6
Predicted MW 62.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_115785
Locus ID 65018
Cytogenetics 1p36.12
Protein Families WB
Protein Pathways Parkinson's disease
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Other "PINK1" proteins (2)
SKU Description Size Price
LC410150 Lyophilized PINK1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP762427 Purified recombinant protein of Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein, Gly349-Pro472, with N-terminal His-ABP tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

Mitochondrial impairment increases FL-PINK1 levels by calcium-dependent gene expression
Gómez-Sánchez, Gegg, Bravo-San Pedro et al
Neurobiol. Dis. (2013)
Cited by 59
AI Summary: OriGene’s pCMV6-Neo vector containing full-length wild type PINK1 cDNA was used to over-express PINK1 in SH-SY5Y cells, enabling comparison with endogenous protein levels in the study.
Snippet: For the over-expression of PINK1 (to compare with the endogenous protein), SH-SY5Y cells were transfected with pCMV6-Neo vector containing full-length wild type PINK1 cDNA (Origene), as previously described (Gegg et al., 2009), using FuGENE 6 Transfection
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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