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PINK1 (NM_032409) Human Over-expression Lysate

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SKU
LC410150
Lyophilized PINK1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206970
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_032409, NP_115785
Synonyms BRPK; PARK6
Predicted MW 62.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_115785
Locus ID 65018
Cytogenetics 1p36.12
Protein Families WB
Protein Pathways Parkinson's disease
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Other "PINK1" proteins (2)
SKU Description Size Price
LY410150 Transient overexpression lysate of PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein (as WB positive control) 100 ug
$436.00
TP762427 Purified recombinant protein of Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein, Gly349-Pro472, with N-terminal His-ABP tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

Mitochondrial impairment increases FL-PINK1 levels by calcium-dependent gene expression
Gómez-Sánchez, Gegg, Bravo-San Pedro et al
Neurobiol. Dis. (2013)
Cited by 59
AI Summary: OriGene’s pCMV6-Neo vector containing full-length wild type PINK1 cDNA was used to over-express PINK1 in SH-SY5Y cells, enabling comparison with endogenous protein levels in the study.
Snippet: For the over-expression of PINK1 (to compare with the endogenous protein), SH-SY5Y cells were transfected with pCMV6-Neo vector containing full-length wild type PINK1 cDNA (Origene), as previously described (Gegg et al., 2009), using FuGENE 6 Transfection
Showing 1-1 of 1 papers.
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