DAB2IP (NM_032552) Human Over-expression Lysate
SKU
LY410041
Transient overexpression lysate of DAB2 interacting protein (DAB2IP), transcript variant 1 (as WB positive control)
$665.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC214413 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_032552, NP_115941 |
| Synonyms | AF9Q34; AIP-1; AIP1; DIP1/2 |
| Predicted MW | 124.9 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_115941 |
| Locus ID | 153090 |
| Cytogenetics | 9q33.2 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "DAB2IP" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH314413 | DAB2IP MS Standard C13 and N15-labeled recombinant protein (NP_115941) | 10 ug |
$3,360.00
|
|
| LC410041 | Lyophilized DAB2IP HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP314413 | Purified recombinant protein of Homo sapiens DAB2 interacting protein (DAB2IP), transcript variant 1, 20 µg | 20 ug |
$867.00
|
|
Citations
The RASSF1A Tumor Suppressor Binds the RasGAP DAB2IP and Modulates RAS Activation in Lung Cancer
Harrell Stewart, Schmidt, Donninger et al
Cancers (Basel) (2020) 12 (12) DOI: 10.3390/cancers12123807
Harrell Stewart, Schmidt, Donninger et al
Cancers (Basel) (2020) 12 (12) DOI: 10.3390/cancers12123807
Cited by 8
AI Summary: OriGene contributed to this study by providing a specific shRNA used to knock down RASSF1A expression in NCI-H1437 lung cancer cells, enabling the authors to investigate the role of RASSF1A in regulating RAS activation.
Snippet: … Knockdown of RASSF1A in NCI-H1792 cells was achieved using an shRNA described in [16], whereas the NCI-H1437 cells express a different shRNA purchased from Origene (described in [40]). … Knockdown of RASSF1A in NCI-H1792 cells was achieved using an shRNA described in [16], whereas the NCI-H1437 cells express a different shRNA purchased from Origene (described in [40]). …
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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