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DAB2IP (NM_032552) Human Over-expression Lysate

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SKU
LC410041
Lyophilized DAB2IP HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC214413
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_032552, NP_115941
Synonyms AF9Q34; AIP-1; AIP1; DIP1/2
Predicted MW 124.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_115941
Locus ID 153090
Cytogenetics 9q33.2
Protein Families WB
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Other "DAB2IP" proteins (3)
SKU Description Size Price
PH314413 DAB2IP MS Standard C13 and N15-labeled recombinant protein (NP_115941) 10 ug
$3,360.00
LY410041 Transient overexpression lysate of DAB2 interacting protein (DAB2IP), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP314413 Purified recombinant protein of Homo sapiens DAB2 interacting protein (DAB2IP), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

The RASSF1A Tumor Suppressor Binds the RasGAP DAB2IP and Modulates RAS Activation in Lung Cancer
Harrell Stewart, Schmidt, Donninger et al
Cancers (Basel) (2020) 12 (12) DOI: 10.3390/cancers12123807
Cited by 8
AI Summary: OriGene contributed to this study by providing a specific shRNA used to knock down RASSF1A expression in NCI-H1437 lung cancer cells, enabling the authors to investigate the role of RASSF1A in regulating RAS activation.
Snippet: … Knockdown of RASSF1A in NCI-H1792 cells was achieved using an shRNA described in [16], whereas the NCI-H1437 cells express a different shRNA purchased from Origene (described in [40]). … Knockdown of RASSF1A in NCI-H1792 cells was achieved using an shRNA described in [16], whereas the NCI-H1437 cells express a different shRNA purchased from Origene (described in [40]). …
FigureFigureFigure
Showing 1-1 of 1 papers.
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