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ERAS (NM_181532) Human Over-expression Lysate

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SKU
LY405694
Transient overexpression lysate of ES cell expressed Ras (ERAS) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210965
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_181532, NP_853510
Synonyms HRAS2; HRASP
Predicted MW 25.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_853510
Locus ID 3266
Cytogenetics Xp11.23
Protein Families WB
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Other "ERAS" proteins (3)
SKU Description Size Price
PH310965 ERAS MS Standard C13 and N15-labeled recombinant protein (NP_853510) 10 ug
$3,360.00
LC405694 Lyophilized ERAS HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP310965 Recombinant protein of human ES cell expressed Ras (ERAS), 20 µg 20 ug
$737.00

file-alt Citations

ERAS Is Constitutively Expressed in the Tissues of Adult Horses and May Be a Key Player in Basal Autophagy
De Falco, Perillo, Del Piero et al
Front Vet Sci (2022) 9, 818294 DOI: 10.3389/fvets.2022.818294
Cited by 3
AI Summary: OriGene products, specifically the anti-human embryonic stem cell-expressed Ras (ERAS) antibody (TA324562) and the ERAS HEK 293T cell transient overexpression lysate and Western blot positive control (LC405694), were used in this study to detect and validate ERAS protein expression in horse tissues, supporting the investigation of ERAS’s physiological role.
Snippet: nst human embryonic stem cell-expressed Ras (ERAS) (TA324562), diluted 1:1000 in BSA, with ERAS HEK 293T cell transient overexpression lysate and WB positive control (COD. LC405694) was obtained from OriGene Technologies, Inc. (MD, USA). This specific antibody was produced against a human ERAS epitope composed of 31 amino acid residues at position 51–81. The human epitope revealed a 100% identit
FigureFigureFigure
WB IHC
Showing 1-1 of 1 papers.
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