ERAS (NM_181532) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | ERAS |
| Synonyms | HRAS2; HRASP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_181532 |
| ORF Size | 699 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_181532.3 |
| RefSeq Size | 826 bp |
| RefSeq ORF | 702 bp |
| Locus ID | 3266 |
| UniProt ID | Q7Z444 |
| Cytogenetics | Xp11.23 |
| Protein Families | Druggable Genome |
| MW | 25.3 kDa |
| Summary | This gene encodes a constitutively active member of the small GTPase Ras protein family. The encoded protein activates the phosphatidylinositol 3-kinase signal transduction pathway in undifferentiated stem cells, but is not expressed in differentiated cells. This gene may be involved in cancer and chemotherapy resistance. provided by RefSeq, Dec 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_181532" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210965L1 | Lenti ORF clone of Human ES cell expressed Ras (ERAS), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC210965L2 | Lenti ORF clone of Human ES cell expressed Ras (ERAS), mGFP tagged | 10 ug |
$600.00
|
|
| RC210965L3 | Lenti ORF clone of Human ES cell expressed Ras (ERAS), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC210965L4 | Lenti ORF clone of Human ES cell expressed Ras (ERAS), mGFP tagged | 10 ug |
$600.00
|
|
| RG210965 | ERAS (tGFP-tagged) - Human ES cell expressed Ras (ERAS) | 10 ug |
$489.00
$500.00
|
|
| SC307300 | ERAS (untagged)-Human ES cell expressed Ras (ERAS) | 10 ug |
$300.00
|
|
Citations
Discovery of novel oestrogen receptor α agonists and antagonists by screening a revisited privileged structure moiety for nuclear receptors
Masuya, Iwamoto, Liu et al
Sci Rep (2019) 9 (1), 9954 DOI: 10.1038/s41598-019-46272-y
Masuya, Iwamoto, Liu et al
Sci Rep (2019) 9 (1), 9954 DOI: 10.1038/s41598-019-46272-y
AI Summary: In this study, the ERα cDNA clone from OriGene Technologies was used to express and purify the glutathione S-transferase (GST)-fused ERα ligand-binding domain, enabling the screening of BPA-related compounds for their binding affinity and activity toward oestrogen receptor α.
Snippet: Dimethyl sulfoxide (DMSO) used to dissolve each compound was obtained from Sigma-Aldrich. Expression and purification of glutathione S‐transferase (GST)-fused ERα LBD. The ERα cDNA clone was purchased from OriGene Technologies, Inc. (Rockville, MD, USA
BRAF inhibitor resistance mediated by the AKT pathway in an oncogenic BRAF mouse melanoma model
Perna, Karreth, Rust et al
PNAS (2015)
Perna, Karreth, Rust et al
PNAS (2015)
Cited by 143
AI Summary: OriGene’s TrueORF human ERAS full-length cDNA was used to express ERAS in melanoma cells, enabling the study of its role in mediating resistance to BRAF inhibitor treatment in this research.
Snippet: Plasmids and Retroviral Infections. The human ERAS full-length cDNA was purchased as a TrueORF (OriGene) and sequence-verified. …
Showing 1-2 of 2 papers.
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