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UBE2D3 (NM_181886) Human Over-expression Lysate

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SKU
LY405574
Transient overexpression lysate of ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) (UBE2D3), transcript variant 2 (as WB positive control)
  • LY405574 is the same product as LY430581.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213585
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_181886, NP_871615
Synonyms E2(17)KB3; UBC4/5; UBCH5C
Predicted MW 16.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_871615
Locus ID 7323
Cytogenetics 4q24
Protein Families WB
Protein Pathways Ubiquitin mediated proteolysis
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Other "UBE2D3" proteins (26)
SKU Description Size Price
PH307371 UBE2D3 MS Standard C13 and N15-labeled recombinant protein (NP_003331) 10 ug
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PH313585 UBE2D3 MS Standard C13 and N15-labeled recombinant protein (NP_871615) 10 ug
$3,360.00
PH313686 UBE2D3 MS Standard C13 and N15-labeled recombinant protein (NP_871618) 10 ug
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PH315841 UBE2D3 MS Standard C13 and N15-labeled recombinant protein (NP_871616) 10 ug
$3,360.00
PH318977 UBE2D3 MS Standard C13 and N15-labeled recombinant protein (NP_871622) 10 ug
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LC405574 Lyophilized UBE2D3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC405575 Lyophilized UBE2D3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC405576 Lyophilized UBE2D3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC405577 Lyophilized UBE2D3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC405578 Lyophilized UBE2D3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC405579 Lyophilized UBE2D3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC405581 Lyophilized UBE2D3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC418758 Lyophilized UBE2D3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY405575 Transient overexpression lysate of ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) (UBE2D3), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY405576 Transient overexpression lysate of ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) (UBE2D3), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY405577 Transient overexpression lysate of ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) (UBE2D3), transcript variant 5 (as WB positive control) 100 ug
$436.00
LY405578 Transient overexpression lysate of ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) (UBE2D3), transcript variant 6 (as WB positive control) 100 ug
$436.00
LY405579 Transient overexpression lysate of ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) (UBE2D3), transcript variant 7 (as WB positive control) 100 ug
$436.00
LY405581 Transient overexpression lysate of ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) (UBE2D3), transcript variant 9 (as WB positive control) 100 ug
$436.00
LY418758 Transient overexpression lysate of ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) (UBE2D3), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP307371 Recombinant protein of human ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) (UBE2D3), transcript variant 1, 20 µg 20 ug
$867.00
TP313585 Purified recombinant protein of Homo sapiens ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) (UBE2D3), transcript variant 2, 20 µg 20 ug
$867.00
TP313686 Purified recombinant protein of Homo sapiens ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) (UBE2D3), transcript variant 5, 20 µg 20 ug
$867.00
TP315841 Recombinant protein of human ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) (UBE2D3), transcript variant 3, 20 µg 20 ug
$867.00
TP318977 Recombinant protein of human ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) (UBE2D3), transcript variant 9, 20 µg 20 ug
$867.00
TP721151 Purified recombinant protein of Human ubiquitin-conjugating enzyme E2D 3 (UBE2D3), transcript variant 1 10 ug
$160.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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