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GPR56 (ADGRG1) (NM_201524) Human Over-expression Lysate

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SKU
LY404437
Transient overexpression lysate of G protein-coupled receptor 56 (GPR56), transcript variant 2 (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC218780
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_201524, NP_958932
Synonyms BFPP; BPPR; GPR56; TM7LN4; TM7XN1
Predicted MW 74.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_958932
Locus ID 9289
Cytogenetics 16q21
Protein Families WB
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Other "GPR56" proteins (11)
SKU Description Size Price
PH300315 GPR56 MS Standard C13 and N15-labeled recombinant protein (NP_005673) 10 ug
$3,360.00
PH318780 GPR56 MS Standard C13 and N15-labeled recombinant protein (NP_958932) 10 ug
$3,360.00
PH326861 GPR56 MS Standard C13 and N15-labeled recombinant protein (NP_001139243) 10 ug
$3,360.00
LC401733 Lyophilized ADGRG1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404437 Lyophilized ADGRG1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC428998 Lyophilized ADGRG1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401733 Transient overexpression lysate of G protein-coupled receptor 56 (GPR56), transcript variant 1 (as WB positive control) 100 ug
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LY428998 Transient overexpression lysate of G protein-coupled receptor 56 (GPR56), transcript variant 4 (as WB positive control) 100 ug
$665.00
TP300315 Recombinant protein of human G protein-coupled receptor 56 (GPR56), transcript variant 1, 20 µg 20 ug
$867.00
TP318780 Recombinant protein of human G protein-coupled receptor 56 (GPR56), transcript variant 2, 20 µg 20 ug
$867.00
TP326861 Recombinant protein of human G protein-coupled receptor 56 (GPR56), transcript variant 4, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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