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GPR56 (ADGRG1) (NM_005682) Human Over-expression Lysate

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SKU
LC401733
Lyophilized ADGRG1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200315
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005682, NP_005673
Synonyms BFPP; BPPR; GPR56; TM7LN4; TM7XN1
Predicted MW 77.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_005673
Locus ID 9289
Cytogenetics 16q21
Protein Families WB
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Other "GPR56" proteins (11)
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LY428998 Transient overexpression lysate of G protein-coupled receptor 56 (GPR56), transcript variant 4 (as WB positive control) 100 ug
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TP300315 Recombinant protein of human G protein-coupled receptor 56 (GPR56), transcript variant 1, 20 µg 20 ug
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TP318780 Recombinant protein of human G protein-coupled receptor 56 (GPR56), transcript variant 2, 20 µg 20 ug
$867.00
TP326861 Recombinant protein of human G protein-coupled receptor 56 (GPR56), transcript variant 4, 20 µg 20 ug
$867.00

file-alt Citations

G protein-coupled receptor 56 regulates matrix production and motility of lung fibroblasts
Yang, Wang, Leng et al
Exp. Biol. Med. (Maywood) (2014)
Cited by 12
AI Summary: OriGene's qSTAR qPCR primer pairs for the Homo sapiens GPR56 gene (NM_005682) were used in this study to measure GPR56 expression levels in lung fibroblasts, enabling the authors to investigate its role in matrix production and cell motility.
Snippet: Resultant cDNAs were subjected to real-time PCR using Real Master Mix (SYBR Green;Tiangen). Primers were qSTAR qPCR primer pairs against Homo sapiens gene GPR56 NM_005682 (Origene, Rockville, MD) and β-actin (Table 2;Invitrogen, Shanghai, China ... Cite SaveSaving
Showing 1-1 of 1 papers.
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