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FOXM1 (NM_202002) Human Over-expression Lysate

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SKU
LY404357
Transient overexpression lysate of forkhead box M1 (FOXM1), transcript variant 1 (as WB positive control)
  • LY404357 is the same product as LY430897.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $665.00
In Stock*
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Other products for "FOXM1 or NM_202002"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211450
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_202002, NP_973731
Synonyms FKHL16; FOXM1A; FOXM1B; FOXM1C; HFH-11; HFH11; HNF-3; INS-1; MPHOSPH2; MPP-2; MPP2; PIG29; TRIDENT
Predicted MW 88.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_973731
Locus ID 2305
Cytogenetics 12p13.33
Protein Families WB
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Other "FOXM1" proteins (8)
SKU Description Size Price
LC404357 Lyophilized FOXM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404358 Lyophilized FOXM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC411864 Lyophilized FOXM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC430897 Lyophilized FOXM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY404358 Transient overexpression lysate of forkhead box M1 (FOXM1), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY411864 Transient overexpression lysate of forkhead box M1 (FOXM1), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY430897 Transient overexpression lysate of forkhead box M1 (FOXM1), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP761439 Purified recombinant protein of Human forkhead box M1 (FOXM1), transcript variant 3, full length, with N-terminal GST and C-terminal HIS tag, expressed in E.coli, 50ug 50 ug
$270.00

file-alt Citations

SOD3 induces a HIF-2α-dependent program in endothelial cells that provides a selective signal for tumor infiltration by T cells
Carmona-Rodríguez, Martínez-Rey, Fernández-Aceñero et al
J Immunother Cancer (2020) 8 (1) DOI: 10.1136/jitc-2019-000432
Cited by 57
AI Summary: In this study, the OriGene pCMV6-Entry/FoxM1-Myc-DDK plasmid (MR210493) was used to overexpress FoxM1 in endothelial cells, enabling the authors to investigate the role of FoxM1 in SOD3-induced T cell infiltration into tumors.
Snippet: … For FoxM1 overexpression, 1G11 cells were transfected with pCMV6-Entry/FoxM1-Myc-DDK (Origene, MR210493) and clones selected by limiting dilution with neomycin (750 µg/mL;Apollo Scientific);by transfection with pCAG-Δ90-GFP (a gift of Anjen Chenn;Addgene no. 26645) and cells selected by cell sorting. For FoxM1 overexpression, 1G11 cells were transfected with pCMV6-Entry/FoxM1-Myc-DDK (Origene, MR210493) and clones selected by limiting dilution with neomycin (750 µg/mL;Apollo Scientific). …
FigureFigureFigure
Showing 1-1 of 1 papers.
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