FOXM1 (NM_202002) Human Tagged ORF Clone
$1,100.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | FOXM1 |
| Synonyms | FKHL16; FOXM1A; FOXM1B; FOXM1C; HFH-11; HFH11; HNF-3; INS-1; MPHOSPH2; MPP-2; MPP2; PIG29; TRIDENT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_202002 |
| ORF Size | 2403 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_202002.2 |
| RefSeq Size | 3641 bp |
| RefSeq ORF | 2406 bp |
| Locus ID | 2305 |
| UniProt ID | Q08050 |
| Cytogenetics | 12p13.33 |
| Protein Families | Transcription Factors |
| MW | 88.4 kDa |
| Summary | The protein encoded by this gene is a transcriptional activator involved in cell proliferation. The encoded protein is phosphorylated in M phase and regulates the expression of several cell cycle genes, such as cyclin B1 and cyclin D1. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_202002" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC211450L1 | Lenti-ORF clone of FOXM1 (Myc-DDK-tagged)-Human forkhead box M1 (FOXM1), transcript variant 1 | 10 ug |
$1,400.00
|
|
| RC211450L2 | Lenti-ORF clone of FOXM1 (mGFP-tagged)-Human forkhead box M1 (FOXM1), transcript variant 1 | 10 ug |
$1,400.00
|
|
| RC211450L3 | Lenti-ORF clone of FOXM1 (Myc-DDK-tagged)-Human forkhead box M1 (FOXM1), transcript variant 1 | 10 ug |
$1,400.00
|
|
| RC211450L4 | Lenti-ORF clone of FOXM1 (mGFP-tagged)-Human forkhead box M1 (FOXM1), transcript variant 1 | 10 ug |
$1,400.00
|
|
| RG211450 | FOXM1 (tGFP-tagged) - Human forkhead box M1 (FOXM1), transcript variant 1 | 10 ug |
$1,300.00
|
|
| SC308090 | FOXM1 (untagged)-Human forkhead box M1 (FOXM1), transcript variant 1 | 10 ug |
$1,101.00
|
|
Citations
Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression in Cancer
Barger, Branick, Chee et al
Cancers (Basel) (2019) 11 (2) DOI: 10.3390/cancers11020251
Barger, Branick, Chee et al
Cancers (Basel) (2019) 11 (2) DOI: 10.3390/cancers11020251
AI Summary: OriGene’s pCMV6 plasmids encoding FOXM1 isoforms a, b, and c were used to express specific FOXM1 variants, enabling the study of their distinct roles in cancer cell models.
Snippet: … The FOXM1 promoter (−2.4 kb) was subcloned from pFlash-FOXM1 [53] into PGL3 to generate PGL3-FOXM1 (−2.4 kb). pCMV6 plasmid (Origene, Rockville, MD, USA) containing FOXM1 isoform a, b, and c was generated with PCR, restriction enzyme digestion and ligation. pCMV6-FOXM1c DNA binding domain mutant (DBD MT) was generated by si;;The FOXM1 promoter (−2.4 kb) was subcloned from pFlash-FOXM1 [53] into PGL3 to generate PGL3-FOXM1 (−2.4 kb). pCMV6 plasmid (Origene, Rockville, MD, USA) containing FOXM1 isoform a, b, and c was generated with PCR, restriction enzyme digestion and ligation
FoxM1 and β-catenin predicts aggressiveness in Middle Eastern ovarian cancer and their co-targeting impairs the growth of ovarian cancer cells
Pratheeshkumar, Divya, Parvathareddy et al
Oncotarget (2018) 9 (3), 3590-3604 DOI: 10.18632/oncotarget.23338
Pratheeshkumar, Divya, Parvathareddy et al
Oncotarget (2018) 9 (3), 3590-3604 DOI: 10.18632/oncotarget.23338
Cited by 21
AI Summary: OriGene products contributed to this study by providing the FoxM1 overexpression plasmid (Cat# RC222278) and FoxM1 siRNA (Cat# SR320763), which were used to manipulate FoxM1 expression levels and investigate its role in regulating β-catenin and ovarian cancer cell growth.
Dual CDK4/6 Inhibition by PD-0332991 induces apoptosis and senescence in esophageal squamous cell carcinoma cells
Chen, Pan
Br. J. Pharmacol. (2017)
Chen, Pan
Br. J. Pharmacol. (2017)
AI Summary: OriGene’s pCMV6-Entry-FOXM1 plasmid was used for transfecting ESCC cells to study the role of FOXM1 in the response to CDK4/6 inhibition by PD-0332991.
Snippet: efficiency. Transfection with plasmid or siRNA ESCC cells were seeded in 6-well flat-bottom plates (Corning) 24 h before tansfection. The empty vector or pCMV6-Entry-FOXM1 (OriGene) plasmid (2.0 µg), and 6 μL linear polyethylenimine transfection reagent (1.0 mg ... All 2 versions Cite SaveSaving
Showing 1-3 of 3 papers.
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