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Myoglobin (MB) (NM_203377) Human Over-expression Lysate

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SKU
LY404319
Transient overexpression lysate of myoglobin (MB), transcript variant 2 (as WB positive control)
  • LY404319 is the same product as LY430914.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213272
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_203377, NP_976311
Synonyms PVALB
Predicted MW 17.2 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_976311
Locus ID 4151
Cytogenetics 22q12.3
Protein Families WB
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Other "Myoglobin" proteins (10)
SKU Description Size Price
PH303113 MB MS Standard C13 and N15-labeled recombinant protein (NP_976312) 10 ug
$3,360.00
PH312352 MB MS Standard C13 and N15-labeled recombinant protein (NP_005359) 10 ug
$3,360.00
LC404319 Lyophilized MB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404320 Lyophilized MB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC417346 Lyophilized MB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY404320 Transient overexpression lysate of myoglobin (MB), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY417346 Transient overexpression lysate of myoglobin (MB), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP303113 Recombinant protein of human myoglobin (MB), transcript variant 3, 20 µg 20 ug
$737.00
TP312352 Recombinant protein of human myoglobin (MB), transcript variant 1, 20 µg 20 ug
$737.00
TP761636 Purified recombinant protein of Human myoglobin (MB), transcript variant 2, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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