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PRAME (NM_006115) Human Over-expression Lysate

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SKU
LY401847
Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 1 (as WB positive control)
  $436.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC214243
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006115, NP_006106
Synonyms CT130; MAPE; OIP-4; OIP4
Predicted MW 57.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_006106
Locus ID 23532
Cytogenetics 22q11.22
Protein Families WB
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Other "PRAME" proteins (13)
SKU Description Size Price
PH307874 PRAME MS Standard C13 and N15-labeled recombinant protein (NP_996838) 10 ug
$3,360.00
LC401847 Lyophilized PRAME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404191 Lyophilized PRAME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404192 Lyophilized PRAME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404193 Lyophilized PRAME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404194 Lyophilized PRAME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY404191 Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY404192 Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 3 (as WB positive control) 100 ug
$665.00
LY404193 Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY404194 Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 5 (as WB positive control) 100 ug
$436.00
TP307874 Purified recombinant protein of Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 4, 20 µg 20 ug
$867.00
TP760464 Purified recombinant protein of Human preferentially expressed antigen in melanoma (PRAME), transcript variant 3, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00
TP760913 Purified recombinant protein of Human preferentially expressed antigen in melanoma (PRAME), transcript variant 5, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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