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PRAME (NM_206955) Human Over-expression Lysate

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SKU
LC404193
Lyophilized PRAME HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207874
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_206955, NP_996838
Synonyms CT130; MAPE; OIP-4; OIP4
Predicted MW 57.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_996838
Locus ID 23532
Cytogenetics 22q11.22
Protein Families WB
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Other "PRAME" proteins (13)
SKU Description Size Price
PH307874 PRAME MS Standard C13 and N15-labeled recombinant protein (NP_996838) 10 ug
$3,360.00
LC401847 Lyophilized PRAME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404191 Lyophilized PRAME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404192 Lyophilized PRAME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404194 Lyophilized PRAME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401847 Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY404191 Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY404192 Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 3 (as WB positive control) 100 ug
$665.00
LY404193 Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY404194 Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 5 (as WB positive control) 100 ug
$436.00
TP307874 Purified recombinant protein of Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 4, 20 µg 20 ug
$867.00
TP760464 Purified recombinant protein of Human preferentially expressed antigen in melanoma (PRAME), transcript variant 3, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00
TP760913 Purified recombinant protein of Human preferentially expressed antigen in melanoma (PRAME), transcript variant 5, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Molecular Insights on Post-chemotherapy Retinoblastoma by Microarray Gene Expression Analysis
Nalini, Segu, Deepa et al
Bioinform Biol Insights (2013) 7, 289-306 DOI: 10.4137/BBI.S12494
AI Summary: OriGene’s PRAME cDNA (NM_206955.1) cloned into the pcDNA3.1 vector was used for transient overexpression experiments to study PRAME’s role in drug response in retinoblastoma cells.
Snippet: as suspension at 37 °C with 5% CO 2 . Transient overexpression of PRAME gene (PRAME cDNA, {"type":"entrez-nucleotide","attrs":{"text":"NM_206955.1","term_id":"46249370"}} NM_206955.1) cloned into the pcDNA3.1 vector was purchased from OriGene Technologies
FigureFigureFigure
Showing 1-1 of 1 papers.
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