BCAT1 (NM_005504) Human Over-expression Lysate
SKU
LY401683
Transient overexpression lysate of branched chain aminotransferase 1, cytosolic (BCAT1) (as WB positive control)
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC219229 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_005504, NP_005495 |
| Synonyms | BCATC; BCT1; ECA39; MECA39; PNAS121; PP18 |
| Predicted MW | 42.8 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_005495 |
| Locus ID | 586 |
| Cytogenetics | 12p12.1 |
| Protein Families | WB |
| Protein Pathways | leucine and isoleucine biosynthesis, leucine and isoleucine degradation, Metabolic pathways, Pantothenate and CoA biosynthesis, Valine |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "BCAT1" proteins (12)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH319229 | BCAT1 MS Standard C13 and N15-labeled recombinant protein (NP_005495) | 10 ug |
$3,360.00
|
|
| LC401683 | Lyophilized BCAT1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC432891 | Lyophilized BCAT1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC432924 | Lyophilized BCAT1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC432972 | Lyophilized BCAT1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC432999 | Lyophilized BCAT1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY432891 | Transient overexpression lysate of branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 3 (as WB positive control) | 100 ug |
$436.00
|
|
| LY432924 | Transient overexpression lysate of branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| LY432972 | Transient overexpression lysate of branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 5 (as WB positive control) | 100 ug |
$436.00
|
|
| LY432999 | Transient overexpression lysate of branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 4 (as WB positive control) | 100 ug |
$436.00
|
|
| TP319229 | Recombinant protein of human branched chain aminotransferase 1, cytosolic (BCAT1), 20 µg | 20 ug |
$867.00
|
|
| TP760653 | Purified recombinant protein of Human branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | 50 ug |
$270.00
|
|
Citations
The BCAT1 CXXC Motif Provides Protection against ROS in Acute Myeloid Leukaemia Cells
Hillier, Allcott, Guest et al
Antioxidants (Basel) (2022) 11 (4) DOI: 10.3390/antiox11040683
Hillier, Allcott, Guest et al
Antioxidants (Basel) (2022) 11 (4) DOI: 10.3390/antiox11040683
Cited by 19
AI Summary: OriGene contributed to this study by providing the BCAT1 cDNA transcript variant 5 in the PCMV6-Entry vector (Cat# PS100001), which was used for site-directed mutagenesis and overexpression experiments to investigate the antioxidant role of the BCAT1 CXXC motif in acute myeloid leukaemia cells.
Snippet: cubated at 37 °C and 5% CO2. 2.2. BCAT1 Site Directed Mutagenesis and Retroviral Transduction of U937 Cells BCAT1 cDNA transcript variant 5 (NM_001178094) contained in PCMV6-Entry vector (PS100001, Origene) was obtained from Origene (Rockville, MD, USA). Site-directed mutagenesis of the BCAT1-CXXC motif was performed using the Quikchange II Site-directed Mutagenesis Kit (Agilent, UK) and mutagen
Showing 1-1 of 1 papers.
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