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MCSF Receptor (CSF1R) (NM_005211) Human Over-expression Lysate

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SKU
LY401594
Transient overexpression lysate of colony stimulating factor 1 receptor (CSF1R) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205288
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005211, NP_005202
Synonyms BANDDOS; C-FMS; CD115; CSF-1R; CSFR; FIM2; FMS; HDLS; M-CSF-R
Predicted MW 107.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005202
Locus ID 1436
Cytogenetics 5q32
Protein Families WB
Protein Pathways Cytokine-cytokine receptor interaction, Endocytosis, Hematopoietic cell lineage, Pathways in cancer
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Other "MCSF Receptor" proteins (6)
SKU Description Size Price
PH305288 CSF1R MS Standard C13 and N15-labeled recombinant protein (NP_005202) 10 ug
$3,360.00
LC401594 Lyophilized CSF1R HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP305288 Recombinant protein of human colony stimulating factor 1 receptor (CSF1R), 20 µg 20 ug
$737.00
TP700112 Purified recombinant protein of human colony stimulating factor 1 receptor, formerly McDonough feline sarcoma viral (v-fms) oncogene homolog (CSF1R), with C-terminal DDK/His tag, expressed in human cells, 20 µg 20 ug
$900.00
TP710105 Recombinant protein of human colony stimulating factor 1 receptor (CSF1R), residues 20-517aa, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
$540.00
TP723975 Recombinant human CSF1R protein with C-terminal 6×His tag 100 ug
$770.00

file-alt Citations

The significance of G-CSF expression and myeloid-derived suppressor cells in the chemoresistance of uterine cervical cancer
Kawano, Mabuchi, Matsumoto et al
Scientific reports (2015)
AI Summary: OriGene contributed to this study by providing the human G-CSFR expression vector (pCMV6-AC-GFP), which was used for transfection experiments to investigate the role of G-CSF signaling in chemoresistance of uterine cervical cancer.
Snippet: Japan. The expression of these genes was driven by the CAG promoter, as reported previously 34 ,35 . The expression vector for the human G-CSFR (pCMV6-AC-GFP) was obtained from OriGene (Rockville, MD). Transfection ... Cite SaveSaving
Showing 1-1 of 1 papers.
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