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MCSF Receptor (CSF1R) (NM_005211) Human Over-expression Lysate

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SKU
LC401594
Lyophilized CSF1R HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205288
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005211, NP_005202
Synonyms BANDDOS; C-FMS; CD115; CSF-1R; CSFR; FIM2; FMS; HDLS; M-CSF-R
Predicted MW 107.8 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_005202
Locus ID 1436
Cytogenetics 5q32
Protein Families WB
Protein Pathways Cytokine-cytokine receptor interaction, Endocytosis, Hematopoietic cell lineage, Pathways in cancer
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Other "MCSF Receptor" proteins (6)
SKU Description Size Price
PH305288 CSF1R MS Standard C13 and N15-labeled recombinant protein (NP_005202) 10 ug
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LY401594 Transient overexpression lysate of colony stimulating factor 1 receptor (CSF1R) (as WB positive control) 100 ug
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TP305288 Recombinant protein of human colony stimulating factor 1 receptor (CSF1R), 20 µg 20 ug
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TP700112 Purified recombinant protein of human colony stimulating factor 1 receptor, formerly McDonough feline sarcoma viral (v-fms) oncogene homolog (CSF1R), with C-terminal DDK/His tag, expressed in human cells, 20 µg 20 ug
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TP710105 Recombinant protein of human colony stimulating factor 1 receptor (CSF1R), residues 20-517aa, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
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file-alt Citations

The significance of G-CSF expression and myeloid-derived suppressor cells in the chemoresistance of uterine cervical cancer
Kawano, Mabuchi, Matsumoto et al
Scientific reports (2015)
AI Summary: OriGene contributed to this study by providing the human G-CSFR expression vector (pCMV6-AC-GFP), which was used for transfection experiments to investigate the role of G-CSF signaling in chemoresistance of uterine cervical cancer.
Snippet: Japan. The expression of these genes was driven by the CAG promoter, as reported previously 34 ,35 . The expression vector for the human G-CSFR (pCMV6-AC-GFP) was obtained from OriGene (Rockville, MD). Transfection ... Cite SaveSaving
Showing 1-1 of 1 papers.
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