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REC8 (NM_005132) Human Over-expression Lysate

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SKU
LY401575
Transient overexpression lysate of REC8 homolog (yeast) (REC8), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200132
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005132, NP_005123
Synonyms HR21spB; REC8L1; Rec8p
Predicted MW 62.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005123
Locus ID 9985
Cytogenetics 14q12
Protein Families WB
Protein Pathways Oocyte meiosis
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Other "REC8" proteins (5)
SKU Description Size Price
PH300132 REC8 MS Standard C13 and N15-labeled recombinant protein (NP_005123) 10 ug
$3,360.00
LC401575 Lyophilized REC8 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC420781 Lyophilized REC8 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY420781 Transient overexpression lysate of REC8 homolog (yeast) (REC8), transcript variant 2 (as WB positive control) 100 ug
$665.00
TP300132 Recombinant protein of human REC8 homolog (yeast) (REC8), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Centromeres are dismantled by foundational meiotic proteins Spo11 and Rec8
Hou, Kyriacou, Thadani et al
Nature (2021) 591 (7851), 671-676 DOI: 10.1038/s41586-021-03279-8
Cited by 29
AI Summary: OriGene's SPO11-myc-DDK plasmid (NM_012444) was used to clone and express the SPO11 protein, enabling the study of its role in centromere dismantlement in this research.
Snippet: SPO11-myc-DDK from an Origene plasmid (NM_012444) was cloned into a pEGFP-N1 vector (a kind gift from P. Meltzer)
Showing 1-1 of 1 papers.
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