REC8 (NM_005132) Human Tagged ORF Clone

SKU
RC200132
REC8 (Myc-DDK-tagged)-Human REC8 homolog (yeast) (REC8), transcript variant 1
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol REC8
Synonyms HR21spB; REC8L1; Rec8p
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200132 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCTACTATCCCAACGTGCTTCAGCGCCACACCGGCTGCTTTGCCACCATCTGGCTGGCGGCGACTC
GCGGCAGCCGGTTGGTGAAGCGCGAATACCTGAGGGTGAATGTGGTGAAAACCTGCGAGGAAATCCTCAA
TTACGTGCTGGTACGAGTGCAACCCCCGCAGCCCGGCCTGCCGCGGCCCCGCTTCTCCCTCTATCTCTCA
GCCCAACTTCAGATCGGTGTGATCCGCGTCTATTCTCAACAATGCCAGTACCTCGTGGAGGACATCCAGC
ACATCTTGGAGCGCCTCCACCGTGCCCAGCTGCAGATCCGAATAGATATGGAGACTGAGCTACCCAGCCT
GCTGCTTCCTAACCACCTGGCCATGATGGAGACCCTAGAAGATGCTCCAGATCCCTTTTTTGGGATGATG
TCTGTGGATCCCAGACTTCCTAGTCCTTTCGATATCCCTCAGATTCGACACCTCTTAGAGGCTGCAATCC
CAGAGAGAGTTGAAGAGATCCCTCCTGAAGTTCCTACAGAGCCCAGGGAGCCAGAGAGGATTCCGGTCAC
TGTGCTGCCACCTGAGGCCATCACGATCCTGGAGGCAGAGCCCATACGGATGCTGGAGATTGAGGGTGAA
CGGGAGCTCCCAGAGGTCAGCCGCCGAGAACTGGACCTGCTGATCGCAGAGGAAGAAGAAGCTATCTTGT
TAGAAATCCCGCGGCTCCCACCTCCAGCTCCTGCAGAGGTGGAAGGAATAGGAGAGGCACTGGGTCCTGA
GGAGCTGAGGCTGACAGGCTGGGAACCTGGGGCCCTACTCATGGAGGTGACCCCCCCGGAGGAGCTGCGT
CTGCCAGCCCCACCCAGCCCAGAGAGGAGGCCCCCAGTCCCCCCACCTCCTCGCCGCCGCCGTCGTCGCC
GGTTACTGTTCTGGGACAAGGAGACTCAGATCTCCCCGGAGAAATTCCAGGAACAACTGCAAACCAGAGC
CCACTGCTGGGAATGTCCTATGGTGCAGCCGCCCGAGAGGACCATCAGAGGCCCTGCGGAGTTGTTCAGA
ACCCCAACTCTCTCTGGCTGGCTACCCCCTGAACTACTGGGTCTCTGGACCCATTGTGCCCAGCCACCCC
CAAAAGCCCTCAGGCGAGAGCTGCCTGAGGAGGCAGCCGCTGAGGAGGAAAGGAGAAAGATTGAAGTTCC
AAGTGAGATTGAGGTCCCGAGGGAGGCCCTGGAGCCCAGTGTTCCCCTTATGGTGTCTTTAGAGATCTCC
CTAGAGGCAGCTGAAGAGGAGAAGTCCCGCATCAGCCTCATCCCACCAGAAGAACGGTGGGCCTGGCCTG
AGGTGGAGGCGCCAGAAGCTCCTGCATTGCCCGTGGTGCCTGAACTCCCTGAGGTGCCCATGGAGATGCC
TTTGGTGCTGCCCCCAGAGCTCGAGCTGCTCTCACTGGAAGCAGTGCACAGGGCAGTGGCACTGGAGCTG
CAGGCTAACAGGGAGCCCGACTTCAGCAGCCTGGTGTCACCTCTCAGCCCCCGCAGGATGGCTGCCCGGG
TCTTCTACCTGCTCCTGGTGCTCTCAGCGCAACAGATTCTTCACGTGAAACAAGAAAAGCCATATGGTCG
CCTCCTGATCCAGCCGGGGCCCAGATTCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200132 protein sequence
Red=Cloning site Green=Tags(s)

MFYYPNVLQRHTGCFATIWLAATRGSRLVKREYLRVNVVKTCEEILNYVLVRVQPPQPGLPRPRFSLYLS
AQLQIGVIRVYSQQCQYLVEDIQHILERLHRAQLQIRIDMETELPSLLLPNHLAMMETLEDAPDPFFGMM
SVDPRLPSPFDIPQIRHLLEAAIPERVEEIPPEVPTEPREPERIPVTVLPPEAITILEAEPIRMLEIEGE
RELPEVSRRELDLLIAEEEEAILLEIPRLPPPAPAEVEGIGEALGPEELRLTGWEPGALLMEVTPPEELR
LPAPPSPERRPPVPPPPRRRRRRRLLFWDKETQISPEKFQEQLQTRAHCWECPMVQPPERTIRGPAELFR
TPTLSGWLPPELLGLWTHCAQPPPKALRRELPEEAAAEEERRKIEVPSEIEVPREALEPSVPLMVSLEIS
LEAAEEEKSRISLIPPEERWAWPEVEAPEAPALPVVPELPEVPMEMPLVLPPELELLSLEAVHRAVALEL
QANREPDFSSLVSPLSPRRMAARVFYLLLVLSAQQILHVKQEKPYGRLLIQPGPRFH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_005132
ORF Size 1641 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005132.3
RefSeq Size 2253 bp
RefSeq ORF 1644 bp
Locus ID 9985
UniProt ID O95072
Cytogenetics 14q12
Domains Rad21_Rec8, Rad21_Rec8_N
Protein Pathways Oocyte meiosis
MW 62.6 kDa
Summary This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
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RC200132L1 Lenti ORF clone of Human REC8 homolog (yeast) (REC8), transcript variant 1, Myc-DDK-tagged 10 ug
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RC200132L3 Lenti ORF clone of Human REC8 homolog (yeast) (REC8), transcript variant 1, Myc-DDK-tagged 10 ug
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RC200132L4 Lenti ORF clone of Human REC8 homolog (yeast) (REC8), transcript variant 1, mGFP tagged 10 ug
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Citations

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