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PFKFB4 (NM_004567) Human Over-expression Lysate

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SKU
LY401448
Transient overexpression lysate of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201573
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004567, NP_004558
Predicted MW 54 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004558
Locus ID 5210
Cytogenetics 3p21.31
Protein Families WB
Protein Pathways Fructose and mannose metabolism
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Other "PFKFB4" proteins (3)
SKU Description Size Price
PH301573 PFKFB4 MS Standard C13 and N15-labeled recombinant protein (NP_004558) 10 ug
$3,360.00
LC401448 Lyophilized PFKFB4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP301573 Recombinant protein of human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4), 20 µg 20 ug
$737.00

file-alt Citations

Targeting the sugar metabolism of tumors with a first-in-class 6-phosphofructo-2-kinase (PFKFB4) inhibitor
Chesney, Clark, Lanceta et al
Oncotarget (2015) 6 (20), 18001-11 DOI: 10.18632/oncotarget.4534
Cited by 45
AI Summary: OriGene products, specifically the PFKFB4 cDNA clone (Cat# RC203307) and PFKFB4 shRNA plasmids (Cat# TL313307), were used in this study to manipulate PFKFB4 expression in cancer cell lines, enabling the researchers to investigate the effects of PFKFB4 inhibition on tumor glucose metabolism and growth.
FigureFigureFigure
Fructose-2,6-bisphosphate synthesis by 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 (PFKFB4) is required for the glycolytic response to hypoxia and tumor growth
Chesney, Clark, Klarer et al
Oncotarget (2014) 5 (16), 6670-86 DOI: 10.18632/oncotarget.2213
Cited by 98
AI Summary: OriGene products contributed to this study by providing PFKFB4 cDNA (OriGene, SC119292) and PFKFB4 shRNA (OriGene, TL311886), which were used to manipulate PFKFB4 expression and investigate its role in glycolysis and tumor growth.
FigureFigureFigure
Showing 1-2 of 2 papers.
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