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PFKFB4 (NM_004567) Human Over-expression Lysate

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SKU
LC401448
Lyophilized PFKFB4 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201573
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004567, NP_004558
Predicted MW 54 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_004558
Locus ID 5210
Cytogenetics 3p21.31
Protein Families WB
Protein Pathways Fructose and mannose metabolism
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Other "PFKFB4" proteins (3)
SKU Description Size Price
PH301573 PFKFB4 MS Standard C13 and N15-labeled recombinant protein (NP_004558) 10 ug
$3,360.00
LY401448 Transient overexpression lysate of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4) (as WB positive control) 100 ug
$436.00
TP301573 Recombinant protein of human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4), 20 µg 20 ug
$737.00

file-alt Citations

Targeting the sugar metabolism of tumors with a first-in-class 6-phosphofructo-2-kinase (PFKFB4) inhibitor
Chesney, Clark, Lanceta et al
Oncotarget (2015) 6 (20), 18001-11 DOI: 10.18632/oncotarget.4534
Cited by 45
AI Summary: OriGene products, specifically the PFKFB4 cDNA clone (Cat# RC203307) and PFKFB4 shRNA plasmids (Cat# TL313307), were used in this study to manipulate PFKFB4 expression in cancer cell lines, enabling the researchers to investigate the effects of PFKFB4 inhibition on tumor glucose metabolism and growth.
FigureFigureFigure
Fructose-2,6-bisphosphate synthesis by 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 (PFKFB4) is required for the glycolytic response to hypoxia and tumor growth
Chesney, Clark, Klarer et al
Oncotarget (2014) 5 (16), 6670-86 DOI: 10.18632/oncotarget.2213
Cited by 98
AI Summary: OriGene products contributed to this study by providing PFKFB4 cDNA (OriGene, SC119292) and PFKFB4 shRNA (OriGene, TL311886), which were used to manipulate PFKFB4 expression and investigate its role in glycolysis and tumor growth.
FigureFigureFigure
Showing 1-2 of 2 papers.
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