YY1 (NM_003403) Human Over-expression Lysate
SKU
LY401166
Transient overexpression lysate of YY1 transcription factor (YY1) (as WB positive control)
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC207198 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_003403, NP_003394 |
| Synonyms | DELTA; GADEVS; INO80S; NF-E1; UCRBP; YIN-YANG-1 |
| Predicted MW | 44.7 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Bioactivity | DNA-binding activity |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_003394 |
| Locus ID | 7528 |
| Cytogenetics | 14q32.2 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "YY1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC401166 | Lyophilized YY1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP721191 | Purified recombinant protein of Human YY1 transcription factor (YY1) | 10 ug |
$340.00
|
|
| TP762172 | Purified recombinant protein of Human YY1 transcription factor (YY1), Gly157-End, with N-terminal His tag, expressed in E.coli, 50ug | 50 ug |
$270.00
|
|
Citations
MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1
Jin, Wu, Wang et al
Stem Cell Res (2016) 17 (2), 277-284 DOI: 10.1016/j.scr.2016.07.011
Jin, Wu, Wang et al
Stem Cell Res (2016) 17 (2), 277-284 DOI: 10.1016/j.scr.2016.07.011
Cited by 33
miR-34a inhibits the migration and invasion of esophageal squamous cell carcinoma by targeting Yin Yang-1
Nie, Ge, Geng et al
Oncol. Rep. (2015)
Nie, Ge, Geng et al
Oncol. Rep. (2015)
Cited by 60
Effect of a Single-Nucleotide Polymorphism in the Cholecystokinin Type A Receptor Gene on Growth Traits in the Hinai-dori Chicken Breed
Rikimaru, Takeda, Uemoto et al
The Journal of Poultry Science (2013) 50 (3), 206
Rikimaru, Takeda, Uemoto et al
The Journal of Poultry Science (2013) 50 (3), 206
Showing 1-3 of 3 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
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